Place 1.0 ml of the Wright-Giemsa Stain (#26149-01) upon the smear, in sufficient quantity to cover the entire surface, for 3-4 minutes. Add 2.0 ml distilled water or Phosphate Buffer, pH 6.5 (#26149-02) and let stand twice as long as in step 1.
How do you make a Wright stain?
- Prepare a film of blood or bone marrow on a microscopic slide and allow to air dry.
- Place the air-dried smear on the slide staining rack, smear side facing upwards.
- Cover the blood film with undiluted staining solution. ...
- Let stand for 2-3 minutes.
- Add approximately equal amount of buffered water (pH 6.5).
What is in Wright-Giemsa stain?
The Wright-Giemsa stain is a modified Romanowsky stain composed of a combination of basic dyes, viz., methylene blue and its oxidative products, azure A and azure B, and an acidic dye, eosin . The stain is used routinely in hematology laboratories to stain peripheral blood and bone marrow aspirate smears.
How does the Wright-Giemsa stain work?
It is classically a mixture of eosin (red) and methylene blue dyes. It is used primarily to stain peripheral blood smears, urine samples, and bone marrow aspirates , which are examined under a light microscope. In cytogenetics, it is used to stain chromosomes to facilitate diagnosis of syndromes and diseases.
How do you make a 10% Giemsa stain?
Make up a 10% Giemsa solution with distilled/deionized water buffered to pH 7.2 . If only one slide is to be stained, you will require about 3 ml of prepared stain. Allow 3 drops of stock Giemsa solution (from the Pasteur pipette) to each millilitre of buffered water to give a 10% solution.
What is the difference between Giemsa and Wright stain?
The main difference between Giemsa stain and Wright stain is that Giemsa stain is used to stain chromosomes to identify chromosome aberrations . But, Wright stain is used to differentiate blood cell types.
What does Wright stain test for?
Light microscopic examination of a peripheral blood smear stained with Wright or Giemsa stain is a rapid, but insensitive, method for diagnosis of ehrlichiosis . The presence of characteristic dark blue or purple staining cytoplasmic inclusions containing bacteria known as morulae in monocytes (E.
Why Giemsa stain is used?
Giemsa stain is used to obtain differential white blood cell counts . It is also used to differentiate nuclear and cytoplasmic morphology of the various blood cells like platelets, RBCs, WBCs. ... This stain is also used in cytogenetics to stain the chromosomes and identify chromosomal aberrations.
What accounts for the largest portion of blood?
The light yellow colored liquid on the top is the plasma , which accounts for about 55 percent of the blood volume and red blood cells is called the hematocrit,or packed cell volume (PCV).
What is retic stain?
A reticulocyte stain measures aggregates of residual ribosomes and mitochondria that form clumped granular material called reticulum . Reticulocytes appear as polychromatophilic cells seen on a Wright- or Wright-Giemsa-stained blood film.
What is the recommended stain for rapid results?
Wright (Wright-Giemsa) stain
It can be used if rapid results are needed, but should be followed up when possible with a confirmatory Giemsa stain, so that Schüffner’s dots can be demonstrated.
What are the steps of Gram staining?
The performance of the Gram Stain on any sample requires four basic steps that include applying a primary stain (crystal violet) to a heat-fixed smear, followed by the addition of a mordant (Gram’s Iodine) , rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone and lastly, counterstaining with ...
What type of stain is used for blood smears?
Blood films are routinely stained with a Romanowsky-type stain (e.g., Wright or Wright-Giemsa) either manually or using an automatic slide stainer. Romanowsky-type stains are composed of a mixture of eosin and oxidized methylene blue (azure) dyes.
What is the procedure for blood staining technique?
Use of Giemsa stain is the recommended and most reliable procedure for staining thick and thin blood films. Giemsa solution is composed of eosin and methylene blue (azure). The eosin component stains the parasite nucleus red, while the methylene blue component stains the cytoplasm blue.
How do you prepare Field stain A and B?
- Fill up two Coplin jars or wide-mouth bottles: ...
- Make blood smear on a clean glass slide and it is dried in the air.
- Fix in methanol for one minute or get Spray ‘Easyfix’.
- Dry in the air.
- Dip fixed smear to Field Stain B (Red Stain) for 5 to 6 seconds.
- Wash in running tap water.
What is Leishman stain procedure?
Leishman Stain is a neutral stain for blood smears which was devised by the British surgeon W. B. Leishman (1865–1926). It consists of a mixture of eosin (an acidic stain), and Methylene blue (a basic stain) in Methyl alcohol and is usually diluted and buffered during the staining procedure.
