SiliCycle offers different types of plates for thin layer chromatography applications: classical TLC, high performance TLC (also called HPTLC) and preparative TLC (PLC). The plate types are selected based on the type of analysis required and the available budget.
What are the three phases of TLC?
There are three components in TLC: (1) the TLC plate (stationary phase), the development solvent (mobile phase), and the sample to be analyzed (solute) . In our experiment the TLC plate consists of a thin plastic sheet covered with a thin layer of silica gel, a portion of the structure of which is shown below.
What is the difference between TLC and preparative TLC?
Preparative thin layer chromatography (PTLC) is used to separate and isolate amounts of material larger than are normal for analytical TLC. ... In preparative TLC, materials to be separated are often applied as long streaks, rather than spots, in the sample application zone.
What is the size of TLC?
The layer thickness is related to the nature of the analysis (analytical or preparative) as well as the performance of the plate (TLC or HPLTC). The most common layer thicknesses are 150 μm (HPTLC plates), 200 – 250 μm (analytical TLC plates) and 500 – 2,000 μm (preparative TLC plates).
What are the three types of backings typically used for a TLC plate?
TLC plates are available with different backings (also called supports): rigid (glass-backed) or flexible sheets (aluminum & plastic-backed) .
What is TLC used for?
TLC is a chromatography technique used to separate non-volatile mixtures . Thin-layer chromatography can be used to monitor the progress of a reaction, identify compounds present in a given mixture, and determine the purity of a substance.
What is TLC principle?
What is the principle of TLC? TCL is based on the principle of separation through adsorption type . The separation relies on the relative empathy of compounds towards the mobile phase and stationary phase.
What does TLC tell you about purity?
Thin Layer Chromatography (TLC) is a separation technique requiring very little sample. It is primarily used to determine the purity of a compound . A pure solid will show only one spot on a developed TLC plate. In addition, tentative identification of the unknown compound can be made through TLC analysis.
How can I get TLC?
They are prepared by mixing the adsorbent, such as silica gel, with a small amount of inert binder like calcium sulfate (gypsum) and water . This mixture is spread as a thick slurry on an unreactive carrier sheet, usually glass, thick aluminum foil, or plastic.
How do you handle TLC plates?
Handle the plates carefully so that you do not disturb the coating of adsorbent or get them dirty. Measure 0.5 cm from the bottom of the plate . Using a pencil, draw a line across the plate at the 0.5 cm mark. This is the origin: the line on which you will spot the plate.
Why silica gel is used in TLC?
Silica gel is by far the most widely used adsorbent and remains the dominant stationary phase for TLC. ... The surface of silica gel with the highest concentration of geminal and associated silanols is favored most for the chromatography of basic compounds because these silanols are less acidic.
Which silica gel is used in TLC?
Chromatographic Systems
Silica gel is the most common stationary phase in TLC and HPTLC of herbicides but reversed-phases (silica gel modified with C 8 , C 18 , e.g., RP-18 W, Nano-Sil C 18 -100, silica gel impregnated with paraffin oil) can also be used.
What is TLC chamber?
Thin layer chromatography (TLC) is an affinity-based method used to separate compounds in a mixture. ... The sample is spotted onto one end of the TLC plate and placed vertically into a closed chamber with an organic solvent (mobile phase).
Can TLC plate be reused?
Silica TLC plates can be reused . I just let all spots run to the top of the plate, then dry the plate, add some new spots and run the TLC again. Saves me from wasting mounds of TLC plates. As long as you don't use a mobile phase that dissolves the silica, you can reuse the plates as many times as you want.
Do polar compounds move slower up TLC plate?
The stronger a compound is bound to the adsorbent , the slower it moves up the TLC plate . Non-polar compounds move up the plate most rapidly (higher Rf value), whereas polar substances travel up the TLC plate slowly or not at all (lower Rf value).
How do you read a TLC plate?
In simple terms, this value is an indication of how far up a TLC-plate a compound has wandered . A high R f -value indicates that the compound has travelled far up the plate and is less polar, while a lower R f -value indicates that the compound has not travelled far, and is more polar.
