Bacteria which resemble the non-transformed will be found on the LB/(-) pGLO plate . These bacteria were removed from the starter plate, did not have any plasmid added to them, and were replated on an LB plate. Thus, they are virtually identical to the non-transformed starter.
What traits or characteristics should the organism have or not have to be sure it will not harm you or the environment?
What traits or characteristics should the organism have (or not have) to be sure it will not harm you or the environment? It should not produce toxins or infect plants or animals .
Which of the traits that you originally observed for E coli did not seem to become altered?
The traits that I originally observed that didn’t seem to become altered were the color and shape of the bacteria . I arrived at the analysis for each trait by comparing the -pGLO plate with the remaining three plates. 2. Of the E.
Why did no bacteria grow on the LB amp plate?
These are the controls. The LB/AMP -plasmid plate should show no growth because the bacteria have not been exposed to the plasmid with ampicillin resistance .
Do you observe some E coli growing on the LB plate that does not contain ampicillin or arabinose?
Do you observe some E. coli growing on the LB plates which do not contain ampicillin/arabinose? Yes . The bacteria that did not receive the plasmid are growing on a plain LB plate.
What does a transformed E coli cell contain that a normal E coli cell does not?
coli cell contain that a normal E. ... coli cells do not grow in the presence of ampicillin . The amp*^ gene allows bacteria to grow when exposed to ampicillin. Transformed cells are resistant to ampicillin.
How would you change the bacteria environment the plate they are growing on?
How would you change the bacteria’s environment to best tell if they are ampicillin resistant? The best test would be to take some of the bacteria growing on the LB plate and streak them on an LB/amp plate. If the bacteria are viable on the LB/amp plate, then they are resistant to ampicillin.
Can a genetically transformed bacteria pass on its acquired traits to its offspring?
It’s a firmly established fact straight from Biology 101: Traits such as eye color and height are passed from one generation to the next through the parents’ DNA. ... Louis has shown that the DNA of bacteria that live in the body can pass a trait to offspring in a way similar to the parents’ own DNA.
What is meant by a control plate?
What is meant by the control plate? ... The control plate does not contain the pGLO plasmid, but the other variables are the same. The control plate proves that the transformation is dependent on the presence of the pGLO plasmid. The LB+amp+ara control plate is missing.
Is it possible to transform an organism?
Transformation is one of three forms of horizontal gene transfer that occur in nature among bacteria, in which DNA encoding for a trait passes from one bacterium to another and is integrated into the recipient genome by homologous recombination; the other two are transduction, carried out by means of a bacteriophage, ...
What happens if you incubate bacteria too long?
If a bacterial culture is left in the same media for too long, the cells use up the available nutrients, excrete toxic metabolites, and eventually the entire population will die . Thus bacterial cultures must be periodically transferred, or subcultured, to new media to keep the bacterial population growing.
What causes bacteria to not grow?
It turns out that stressful conditions cause some proteins to be literally bent out of shape, or misfolded, and they stop working, says Chien. ... Specifically, we’ve shown that certain kinds of bacteria respond to high temperatures by destroying proteins needed for DNA replication . Therefore, they stop growing.
What is required for growth on the LB amp plate?
And, in the LB/amp plate, the – DNA cells will not grow because they have not been transformed into ampicillin-resistant cells. ... = The cell would have to be transformed in order to obtain the gene for fluorescence. Then, the gene needs to be “turned on” by the arabinose sugar .
Why does UV light make pGLO glow?
“The beta-lactamase protein is produced and secreted by bacteria that contain the plasmid. ... The Green Fluoresce Protein (GFP) is a gene code that is inserted into the pGLO plasmid to make it glow green under UV light with the help of the araC gene.
Which protein is responsible for allowing the bacteria to grow in the presence of ampicillin?
The 10-minute incubation period following the addition of LB nutrient broth allows the cells to grow and express the ampicillin resistance protein beta-lactamase , so that the transformed cells survive on the subsequent ampicillin selection plates.
Why is arabinose added to the growth media?
Addition of arabinose sugar to the growth media will cause RNA polymerase to start transcribing the GFP gene (i.e. making mRNA molecules) . The cellular machinery (e.g. ribosomes) will translate this mRNA into corresponding GFP protein.
