Does Real Timepcr Use Annealing And Extending Cycles?

Does Real Timepcr Use Annealing And Extending Cycles? Real-time PCR permits the identification of specific, amplified DNA fragments using analysis of their melting temperature (also called Tm value, from melting temperature). The method used is usually PCR with double-stranded DNA-binding dyes as reporters and the dye used is usually SYBR Green. How many cycles are