The main test for authenticating cell lines is short tandem repeat (STR) profiling, the analysis of microsatellite regions of DNA that have variable numbers of repeats and are located throughout the genome . ... STR profiling is the test that most journals require, says Eterovic, and the assay is relatively simple.
How do you check cell lines?
The experts guidelines and recommendations say that the best way to identify cell lines is making a STR (Short Tandem Repeats) allele profile and compare it to well established databases .
What are cell lines used for?
Cell lines have revolutionized scientific research and are being used in vaccine production , testing drug metabolism and cytotoxicity, antibody production, study of gene function, generation of artificial tissues (e.g., artificial skin) and synthesis of biological compounds e.g., therapeutic proteins.
What is the cell line authentication?
Cell lines are fundamental research tools that serve as models for complex biological systems, especially those of human tissue. ... A widely used method for cell line authentication involves STR profiling, a PCR-based technique that compares multiple short tandem repeat (STR) markers between two or more cellular genomes .
Why is cell line authentication important?
Cell and microbe authentication matters
Cell and microbial authentication is of the utmost importance; skipping the authentication step can lead to lost time and money and retractions of publication as cross-contamination or misidentification has invalidated the data that that research is built upon.
What are the types of cell lines?
- Frog cell lines.
- Hamster cell lines.
- Mouse cell lines.
- Rat cell lines.
- Dog cell lines.
How do you develop cell lines?
Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein . The first step in the process is the isolation of single, viable cells. Limiting dilution is a technique that relies on statistical probability but is time consuming.
What is the difference between primary cells and cell lines?
Primary cell culture is the culture of cells directly isolated from parental tissue of interest; whereas cell line is the culture of cells originated from a primary cell culture, which is generally used to expand cell population and prolong life span.
What is HEK293 cell line?
HEK293 is a hypotriploid human cell line . The modal HEK293 chromosome number is 64 in 30% of cells, with higher ploidy occurring in 4.2% of cells. They have three copies of the X chromosome, and a 4 kbp fragment of Ad5 was incorporated into chromosome 19 of the HEK293 genome, which displays cytogenetic instability.
What is cellular Confluency?
Confluency is the percentage area covered by adherent cells . This measurement is routinely used to monitor cell growth and expansion during cell culture experiments. It is key in determining the optimal timings for cell harvest, passage and process interventions such as drug treatment or cell differentiation.
What is a cell line in cell culture?
Cell Line. A cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space . Lines differ from cell strains in that they become immortalized.
What should be done to assure reliability of cell lines used?
Once a cell line has been developed or acquired and validated (i.e., shown to be authentic and uncontaminated) the first step to ensuring a reliable and reproducible supply of cells is the cryopreservation of about 20 1-ml ampoules, each containing 1–5 × 10 6 cells .
What is STR analysis used for?
STR analysis is a tool in forensic analysis that evaluates specific STR regions found on nuclear DNA . The variable (polymorphic) nature of the STR regions that are analyzed for forensic testing intensifies the discrimination between one DNA profile and another.
What is the cell line?
Cell line. (Science: cell culture) a cell line is a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space . lines differ from cell strains in that they have escaped the Hayflick limit and become immortalised.
What are permanent cell lines?
Permanent cells are defined as cells that are unable to replicate in postnatal life . Nervous cells, also termed neurons, together with skeletal muscle and cardiac cells, are included in this group, which traditionally identifies the human tissues that are incapable of spontaneous regeneration.
What are human cell lines?
Human cell lines are immortalized cells propagated in vitro from primary explants of human tissue or body fluid . The use of human cell cultures as a model for more complex biological systems is an integral part of molecular biology, and biomedical research.
