Wright’s stain is a hematologic stain that facilitates the differentiation of blood cell types. It is classically a mixture of eosin (red) and methylene blue dyes. It is used primarily to stain peripheral blood smears, urine samples, and bone marrow aspirates , which are examined under a light microscope.
What is the use of Giemsa stain?
Giemsa stain is performed on paraffin sections. It is used to stain the blood cells of hematopoietic tissues . It can also be applied to all tissue sections in which the presence of microorganisms is suspected. Gram + and Gram Bacteria are not differentiated with this staining.
What is the difference between Giemsa stain and Wright stain?
The main difference between Giemsa stain and Wright stain is that Giemsa stain is used to stain chromosomes to identify chromosome aberrations . But, Wright stain is used to differentiate blood cell types.
How do you use Wright’s blood stain?
- Place 1.0 ml of the Wright Stain Solution upon the smear 1 – 3 minutes.
- Add 2.0 ml distilled water or Phosphate buffer pH 6.5 and let stand twice as long as in step 1.
- Rinse stained smear with water or the Phosphate buffer pH 6.5 until the edges show faintly pinkish-red.
What is the purpose of buffer in Wright’s staining?
Proper buffer selection is very important in achieving good quality staining . If a buffer is too acidic the stain will be too red and nuclei will be too light; if it is too basic the stain will be too blue and cytoplasmic detail will be indistinct.
Can Giemsa stain bacteria?
Giemsa stains the fungus Histoplasma, Chlamydia bacteria , and can be used to identify mast cells.
Why Giemsa stain is used in G banding?
G-banding, G banding or Giemsa banding is a technique used in cytogenetics to produce a visible karyotype by staining condensed chromosomes . ... Heterochromatic regions, which tend to be rich with adenine and thymine (AT-rich) DNA and relatively gene-poor, stain more darkly in G-banding.
What is a Wright stain and what does it stain?
Wright’s stain is a polychromatic stain consisting of a mixture of eosin and methylene Blue . When applied to blood cells, the dyes produce multiple colors based on the ionic charge of the stain and the various components of the cell.
What is Field stain A and B?
Field stain is a histological method for staining of blood smears. It is used for staining thick blood films in order to discover malarial parasites. ... Field’s stain consists of two parts – Field’s stain A is methylene blue and Azure 1 dissolved in phosphate buffer solution ; Field’s stain B is Eosin Y in buffer solution.
How do you use Field stain A and B?
- Fill up two Coplin jars or wide-mouth bottles: ...
- Make blood smear on a clean glass slide and it is dried in the air.
- Fix in methanol for one minute or get Spray ‘Easyfix’.
- Dry in the air.
- Dip fixed smear to Field Stain B (Red Stain) for 5 to 6 seconds.
- Wash in running tap water.
How do you stain Supravital?
The most common supravital stain is performed on reticulocytes using new methylene blue or brilliant cresyl blue, which makes it possible to see the reticulofilamentous pattern of ribosomes characteristically precipitated in these live immature red blood cells by the supravital stains.
What is the principle of Leishman stain?
Leishman stain contain methylene blue dye, a basic dye, which gives colour to an acidic component and eosin dye, an acidic dye, which gives colour to a basic component. These dyes differentiate the different component of blood. Leishman’s Stain belongs to the group of Romanowsky stains.
Which stain is used for Pap smear?
Papanicolaou stain (also Papanicolaou’s stain and Pap stain) is a multichromatic (multicolored) cytological staining technique developed by George Papanicolaou in 1942. The Papanicolaou stain is one of the most widely used stains in cytology, where it is used to aid pathologists in making a diagnosis.
How do you store Wright stains?
Storing, stability and expiry date
Keep Wright’s stain in a tightly closed original package at temperature between 15°C and 25°C.
How does Diff Quik stain work?
The Diff-Quik stain consists of a fixative agent (methanol, blue), solution I (eosinophilic, orange) and solution II (basophilic, blue). Generally, slides are dipped sequentially into each solution 6 times (or left for 10-15 seconds in each solution), followed by a water rinse and drying .
How does Giemsa stain work?
Giemsa solution is composed of eosin and methylene blue (azure). The eosin component stains the parasite nucleus red, while the methylene blue component stains the cytoplasm blue . The thin film is fixed with methanol. De-haemoglobinization of the thick film and staining take place at the same time.
