CB17 SCID mouse (SCID means severe combined immunodeficiency) is
an albino strain with a spontaneous SCID mutation
. The mutation prevents the development and maturation of both T and B cells.
What is an inbred mouse strain?
Inbred mouse strains are defined as
colonies produced by a minimum of 20 generations of brother-sister mating
, traceable to a single founding pair.
What does mouse strain mean?
The name of a mouse strain
reflects the unique phenotypic and genetic characteristics of widely used laboratory mice
. The nomenclature of a strain may have punctuation marks, italics, superscripts, and various symbols – all of which mean something.
How do you write a genotype for a mouse?
For spontaneously or targeted mutant mice, this notation makes perfect sense: “
+”
indicates the wildtype allele, and “-” the mutant allele. Thus, we designate our mouse genotypes “-/-” for homozygous mutants, “+/-” for heterozygotes, and “+/+” for wildtypes.
Are inbred mice truly genetically identical?
Inbred laboratory mice provide the possibility to do this. Individual mice within an inbred mutant strain are
essentially genetically identical to each other except for the mutant gene being studied
.
How do you read a mouse strain?
Congenic strains are designated by a compound symbol consisting of the host strain (usually abbreviated), a period, the donor strain (also usually abbreviated), a hyphen, and the italicized symbol of the differential locus or loci and allele(s) (e.g., B10. 129P-H12
b
).
What is the difference between inbred and outbred mice?
Genetically, there are two major classes of laboratory mice: inbred and outbred. Inbred mice are genetically homogeneous and there
is very little variation or heterogeneity within a pure inbred strain
. … Outbred mice are bred specifically to maximize genetic diversity and heterozygosity within a population.
How can you tell if someone is inbred?
- Reduced fertility both in litter size and sperm viability.
- Increased genetic disorders.
- Fluctuating facial asymmetry.
- Lower birth rate.
- Higher infant mortality and child mortality.
- Smaller adult size.
What happens if mice inbreed?
The most important practical consequence of inbreeding is that
there should be virtually no genetic segregation within the strain
and, as such, every mouse is essentially a genetically identical clone of its parents and siblings, allowing for the perpetual propagation of genetically identical animals.
Do mice suffer from inbreeding?
Mice have
been inbred to reduce genetic variance for over
a 100 years, and each generation of inbreeding is expected to lead to a decrease in heterozygosity (Wright 1921; Silver 1995).
Why do we genotype mice?
An essential step in mouse breeding is
genotyping your mice
to ensure that they have the expected genotype. The genotype for a specified gene in a mouse can be homozygous, heterozygous, wild type, and sometimes hemizygous (such as X-linked genes or transgenes).
What is a Flox mice?
Htt flox/flox mice – these mice have
two loxP sites inserted
so they border from both sides Htt gene (or usually just the most important exon), loxP sites are 48 bp long DNA sequences recognized by Cre recombinase from bacteriophage P1 of E. coli.
How are lab mice named?
Laboratory mice
are usually of the species Mus musculus. They are the most commonly used mammalian research model and are used for research in genetics, psychology, medicine and other scientific disciplines.
Do mice breed with their siblings?
4) Mice that have been housed alone or in same-sex groups
will usually not breed successfully if
they are older than 6-8 months. 5) Occasionally, older mice may breed successfully, but they may have significant problems with the delivery of live pups and nursing.
Are Inbreds deformed?
Inbred people are depicted as
psychotic
, physically deformed individuals who are, more often than not, cannibals living in the Southern United States.
Is line breeding the same as inbreeding?
What is inbreeding? Inbreeding is the mating of related individuals that have one or more relatives in common. Linebreeding is
a form of inbreeding
.
