A Stage Micrometer is simply
a microscope slide with a finely divided scale marked on the surface
. The scale is of a known true length and is used for calibration of optical systems with eyepiece graticule patterns.
What is the role of the stage micrometer in this lab?
The stage micrometer is
used to calibrate an eyepiece reticle when making measurements with a microscope
. Eyepiece Reticle (or reticule) -a small piece of glass with a ruler etched into it that fits into a microscope eyepiece. This ruler is used to make measurements of objects viewed through the microscope.
What is a stage micrometer quizlet?
What is a stage micrometer? What is it used for? Circular disk mounted on a glass slide uniformly spaced lines of known distance.
Used to calibrate a micrometer
. Briefly explain how you calibrate a microscope in order to measure bacteria.
What is the difference between a stage micrometer and an ocular micrometer?
The distance between the lines of an ocular micrometer is an arbitrary measurement that only has meaning if the ocular micrometer is calibrated for the objective being used. A stage micrometer, also known as an objective micrometer, has scribed lines on it that are exactly
0.01mm (10 micrometers)
apart.
What is an ocular micrometer quizlet?
Ocular Micrometer.
Used to measure the size of objects
.
How long is a stage micrometer?
A stage micrometer is simply a microscope slide with a scale etched on the surface. A typical micrometer scale is
2 mm long
and at least part of it should be etched with divisions of 0.01 mm (10 μm). Suppose that a stage micrometer scale has divisions that are equal to 0.1 mm, which is 100 micrometers (μm).
How do you convert micrometer to magnification?
- Measure the scale bar image (beside drawing) in mm.
- Convert to μm (multiply by 1000).
- Magnification = scale bar image divided by actual scale bar length (written on the scale bar).
What is the purpose of an eyepiece reticle?
An eyepiece reticle is a small piece of glass with a ruler or grid imposed on it that fits into the microscope eyepiece. When looking through the microscope, the reticle image is imposed upon your specimen image. Most often the reticle is used
to make measurements or count particles
.
What is the purpose of using an ocular micrometer?
An ocular micrometer is a glass disk that fits in a microscope eyepiece that has a ruled scale, which is used
to measure the size of magnified objects
. The physical length of the marks on the scale depends on the degree of magnification.
Does the ocular micrometer change in magnification?
Note that at different magnifications, the stage micrometer changes, but
the ocular micrometer is fixed in dimension
. In reality, the stage micrometer is also fixed, and what is changing is the power of the magnification of the objective.
What is the purpose of calibrating an ocular micrometer with a stage micrometer?
In order to determine the precise distance between the lines of an ocular micrometer
, it must be calibrated with a stage micrometer (Fig. 2-2). The inscribed lines on a stage micrometer are exactly 0.01 mm (or 10 m) apart.
What are ocular units?
One ocular unit is
the smallest unit visible on the scale
. There are 100 of these in total. Using the mechanical stage controls, line up the left-hand (zero) end of the ocular scale with the left-hand (zero) end of the 2 mm stage scale.
Why should the ocular micrometer be calibrated with a stage micrometer quizlet?
Why is it necessary to calibrate the ocular micrometer with each objective? Each microscope?
The magnification is different for each objective lens
. The numerical aperture value holds only for the specific objective ocular lens combination.
How did you calculate the diameter of the field of view at 400x total magnification?
For example, if the eyepiece reads 10x/18, and the magnification of your objective lens is 40, multiply 10 and 40 to get 400. Then
divide 18 by 400
to get an FOV diameter of 0.045 millimeters.
