Blood clotting time: 60 minutes. Thrombin-based clot activator with gel for serum separation 5 to 6 For stat serum determinations in chemistry. Tube inversions ensure mixing of clot activator with blood.
What is the function of clot activators?
Clot activator
promotes blood clotting with glass or silica particles
. Fluoride inhibits glycolysis, and oxalate prevents clotting by precipitating calcium.
What is the clot activator in serum tubes?
Clot activator
promotes blood clotting with glass or silica particles
. Gel separates serum from cells. Fluoride inhibits glycolysis, and oxalate prevents clotting by precipitating calcium.
How many times should a clot activator tube be inverted?
Tubes should be inverted
5 times
to expose blood to the clot activator. To produce a solid separation barrier, allow the blood to clot in an upright position for at least 30 minutes, but no longer than 1 hour before centrifugation.
What are some clot activators?
Clot activators –
glass, silica, kaolin, bentonite, and diatomaceous earth
– work by surface dependent mechanism whereas extrinsic biomolecules – thrombin, snake venoms, ellagic acid, and thromboplastin – start in vitro coagulation when added to blood.
What is the difference between plasma and serum?
Serum and plasma both come from the liquid portion of the blood that remains once the cells are removed, but that’s where the
similarities
end. Serum is the liquid that remains after the blood has clotted. Plasma is the liquid that remains when clotting is prevented with the addition of an anticoagulant.
Is heparin an anticoagulant?
Anticoagulants such as heparin or warfarin (also called Coumadin)
slow down your body’s process of making clots
. Antiplatelet drugs, such as aspirin, prevent blood cells called platelets from clumping together to form a clot.
What additive is in the yellow tube?
STOPPER COLOR CONTENTS USES/COMMENTS | Green Sodium heparin(100 USP Units) Ammonia, Lactate, HLA Typing | Tan K 2 EDTA Lead levels | Yellow ACD Solution A consists of trisodium citrate, citric acid and dextrose DNA Studies, HIV Cultures | Pink (K 2 )EDTA Blood type & Screen, Compatibility Study, Direct Coombs HIV Viral Load |
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What is a white top tube used for?
WHITE TOP:
This tube type contains EDTA (ethylenediamintetra-acetate) K2 and is used for
collection of HIV Viral Load specimens
. It may also be used for collection of specimens for Hepatitis C PCR testing (both Qualitative and Quantitative) and HIV-1 Genotyping. This tube is available in a 5 mL size.
How many times can you invert tubes?
All tubes (except red top tubes which contain no additives) must be gently inverted
5 to 8 times immediately after filling
, to ensure proper mixing of blood and anticoagulant, or other additives.
Which test would not be performed on plasma or serum?
EDTA
plasma samples are not generally used in the chemistry laboratory because of interference with calcium, electrolytes, iron, magnesium, and alkaline phosphatase assays as noted in the WHO document.
What happens if you spin blood before it clots?
If specimen is centrifuged before clotting is complete,
a fibrin clot will form on top of the cell
. This finding is frequent in hemolyzed specimens. Also, the gel barrier may not be intact and could cause improper separation of serum and cells, possibly affecting test results.
What is the meaning of clot activator?
Clot activator
promotes blood clotting with glass or silica particles
. Gel separates serum from cells.
How many types of anticoagulants are there?
Anticoagulants may be divided into
four main groups
: coumarins and indandiones; factor Xa inhibitors; heparins; and direct thrombin inhibitors.
Is EDTA an anticoagulant?
Historically, EDTA has been recommended as the
anticoagulant
of choice for hematological testing because it allows the best preservation of cellular components and morphology of blood cells.
Why serum is preferred over plasma?
In general, serum samples (red top tubes) are preferred for chemistry testing. This is because our
chemistry reference intervals are based on serum not plasma
. … For example, LDH, potassium and phosphate are higher in serum than plasma, because of release of these constituents from cells during clotting.