What Does Annealing Temperature Depend On?
What Does Annealing Temperature Depend On? The annealing temperature of a standard PCR protocol is either 55°C [2, 3] or 60°C [4]. The chosen temperature depends on the strand-melting temperature of the primers and the desired specificity. For greater stringency higher temperatures are recommended [2]. How does temperature affect annealing? All Answers (3) At the