differential staining

Is Crystal Violet A Simple Stain?

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Is Crystal Violet A Simple Stain? Basic stains, such as methylene blue, Gram safranin, or Gram crystal violet are useful for staining most bacteria. These stains will readily give up a hydroxide ion or accept a hydrogen ion, which leaves the stain positively charged. Is crystal violet a basic dye? The gram stain utilizes crystal

What Are The Steps Of Gram Staining?

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What Are The Steps Of Gram Staining? The performance of the Gram Stain on any sample requires four basic steps that include applying a primary stain (crystal violet) to a heat-fixed smear, followed by the addition of a mordant (Gram’s Iodine), rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone and lastly,