How Much Plasmid Do I Need For Transformation?
How Much Plasmid Do I Need For Transformation? 5-10 ng of plasmid/cosmid DNA are required. 1-For transformation, you could use very less DNA, 1 ng. You need to run a positive transformation control and calculate your transformation efficiency. How much DNA do you need for transformation? For successful chemical transformation, 50–100 μL of competent cells