How Do You Inactivate RNase A?
How Do You Inactivate RNase A? RNase A can be dissolved at a concentration of 1 to 10 mg/ml in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl, heated to 100°C for 15 minutes to inactivate contaminating DNases and cooled slowly to room temperature and dispense into aliquots. Does UV inactivate RNase? UV light can