eukaryotic genes

Why Is It Important To Use A CDNA Rather Than A Genomic Clone When You Want To Express A Eukaryotic Gene In E Coli?

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Why Is It Important To Use A CDNA Rather Than A Genomic Clone When You Want To Express A Eukaryotic Gene In E Coli? It would be necessary to use cDNA so that the gene would not carry any introns. Bacterial cells do not contain spliceosomes (which are described in Chapter 12). To express a

What Is The Core Enzyme In RNA Polymerase?

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What Is The Core Enzyme In RNA Polymerase? The Escherichia coli RNA polymerase (RNAP) is a multi-subunit enzyme composed of five subunits including α (two copies), β, β’ and ω subunits. These five subunits form the RNAP core enzyme responsible for RNA synthesis using DNA as template and ribonucleotide (rNTP) as substrate. Which of the