extinction coefficient

Is The Extinction Coefficient The Same For All Wavelengths?

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Is The Extinction Coefficient The Same For All Wavelengths? Molar extinction coefficient exists for each and every wavelength. The normal practice is to specify the wavelength (say point B, the maximum) with the molar extinction coefficient. How is the extinction coefficient of other wavelengths calculated? According to Beer’s law, A = εbc, where A is

How Do You Calculate Molar Extinction Coefficient From Absorbance?

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How Do You Calculate Molar Extinction Coefficient From Absorbance? According to Beer’s law, A = εbc, where A is the absorbance, ε is the molar extinction coefficient, b is the path length of the cuvette and c is the concentration. Thus, the molar extinction coefficient can be obtained by calculating the slope of the absorbance