fluorescence detection

Why Is Absorbance Better Than Fluorescence?

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Why Is Absorbance Better Than Fluorescence? While absorbance is quick and easy and doesn’t require the preparation or purchase of an assay, fluorescence assays are highly specific for the targeted analyte so that measurement accuracy is not affected by contaminants in a sample. Is fluorescence more accurate than absorbance? Sensitivity: The sensitivity of fluorescence detection

Does Real Timepcr Use Annealing And Extending Cycles?

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Does Real Timepcr Use Annealing And Extending Cycles? Real-time PCR permits the identification of specific, amplified DNA fragments using analysis of their melting temperature (also called Tm value, from melting temperature). The method used is usually PCR with double-stranded DNA-binding dyes as reporters and the dye used is usually SYBR Green. How many cycles are