fluorescence microscopy

Why Is Confocal Microscopy Better Than Fluorescence Microscopy?

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Why Is Confocal Microscopy Better Than Fluorescence Microscopy? Confocal microscopy offers several distinct advantages over traditional widefield fluorescence microscopy, including the ability to control depth of field, elimination or reduction of background information away from the focal plane (that leads to image degradation), and the capability to collect serial optical … What is the difference

What Else Is In The Samples That Might Generate Background Fluorescence?

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What Else Is In The Samples That Might Generate Background Fluorescence? What else is in the samples that might generate background fluorescence? Background fluorescence includes any signal detected beyond what is generated by the fluorochromes being measured. The three major sources of background fluorescence include autofluorescence, spectral overlap, and undesirable antibody binding. What causes background