Can You Purify An Endogenous Protein Using IMAC?
Can You Purify An Endogenous Protein Using IMAC? IMAC is a widely-used method for rapidly purifying polyhistidine affinity-tagged proteins, resulting in 100-fold enrichments in a single purification step. The chelators most commonly used as ligands for IMAC are nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA). What is Ni-NTA chromatography used for? Ni-NTA Agarose is an