What Are The 2 Steps In Two Dimensional 2D Gel Electrophoresis And On What Basis Are Proteins Separated In Each 4 Points?
What Are The 2 Steps In Two Dimensional 2D Gel Electrophoresis And On What Basis Are Proteins Separated In Each 4 Points? 2-DE separates proteins depending on two different steps: the first one is called isoelectric focusing (IEF) which separates proteins according to isoelectric points (pI); the second step is SDS-polyacrylamide gel electrophoresis (SDS-PAGE) which