What Synthesizes The Lagging Strand Of DNA?

What Synthesizes The Lagging Strand Of DNA? Helicase, primase, and two molecules of DNA polymerase III carry out coordinated synthesis of both the leading and lagging strands of DNA. Which polymerase synthesizes the lagging strand? DNA polymerase III lets go of the lagging-strand template after adding about 1000 nucleotides. A new loop is then formed,