How Long Lentivirus Life Cycle?

How Long Lentivirus Life Cycle? The half-life in culture medium ranged from 8 to 40 h for the different envelope-pseudotyped vectors, with 35 h for VSV-G-envelope-pseudotyped vector particles. How long does it take to make lentivirus? All lentiviral vectors present in the transduction mix need at least 5 hours to penetrate the cells of interest.