phase contrast

Why Do You Use Kohler Illumination?

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Why Do You Use Kohler Illumination? The primary advantage of Köhler illumination is the uniform illumination of the sample. This reduces image artifacts and provides high sample contrast. Uniform illumination of the sample is also critical for advanced illumination techniques such as phase contrast and differential interference contrast microscopy. What is the point of Köhler

What Is The Difference Between Brightfield And Phase Contrast Microscopy?

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What Is The Difference Between Brightfield And Phase Contrast Microscopy? An amplitude specimen decreases the intensity (i.e. the amplitude) of the light. Phase specimens cause a phase shift of the light. Phase contrast microscopy is now capable of converting a difference in refractive index into a difference in brightness. … What is the main difference