What Is Plaque Assay Used For? Plaque assays are used to count infectious particles. Samples are diluted and aliquots of each dilution are added to cultured cells. The cells are covered with an agaroseoverlay. Virus produced from an infected cell can infect nearby cells. What do plaque assays detect? Plaque assay Viral plaque assays determine
How Do You Calculate Plaque Forming Units? Divide the number of plaques by the dilution factor, (ex. 10– 6 for the most diluted sample) toobtain the number of Plaque Forming Units (PFU) in 100 μL of phage mixture. What is meant by plaque forming units? A measure of viable bacteriophage particles, determined by plating a
What Is A Bacteriophage Plaque? 1 Introduction. A phage plaque is a clearing in a bacterial lawn. Plaques form via an outward diffusion of phage virions that is fed by bacterial infection. Anything that slows phage diffusion can impede plaque development and thereby plaque size. What is a plaque in microbiology? Plaque, in microbiology, a