Do You Need To Linearize Repair Plasmid?
Do You Need To Linearize Repair Plasmid? So, yes, you should use linearized plasmid to run the gel because the DNA size markers are linear form too. How do you linearize a plasmid? Linearize the shuttle plasmid with either PmeI, NheI, SwaI, or SfiI. Make sure the enzyme you choose does not cut in your