How Do I Cut His Tags?
How Do I Cut His Tags? Dialyze the protein against 20 mM Tris-HCl, pH 7.5. Determine the protein concentration. Combine 15 μg of protein and H2O (if necessary) to make a 45 μl total reaction volume. Add 5 μl of TEV Protease Reaction Buffer (10X) to make a 50 μl total reaction volume. What is