- Recognition of initiation point.
- Unwinding of DNA –
- Template DNA –
- RNA Primer –
- Chain Elongation –
- Replication forks –
- Proof reading –
- Removal of RNA primer and completion of DNA strand –
What are the 6 enzymes involved in DNA replication?
Helicase (unwinds the DNA double helix) Gyrase (relieves the buildup of torque during unwinding) Primase (lays down RNA primers)
DNA polymerase III
(main DNA synthesis enzyme)
What are the steps of DNA replication in order quizlet?
- Step 1: Starts at? DNA Replication begins at the Origin of Replication.
- Step 2: Unwinds. …
- Step 3: Holds strands. …
- Step 4: Two types of strands added 3′ to 5′ …
- Step 5: RNA Primer. …
- Step 6: Add bases. …
- Step 7: Fix mistakes, remove RNA Primer. …
- Step 9: join fragments together.
What is the process of DNA replication?
DNA replication is the
process by which a double-stranded DNA molecule is copied to produce two identical DNA molecules
. … Once synthesis is complete, the RNA primers are replaced with DNA, and any gaps between newly synthesized DNA segments are sealed together with enzymes.
What are the steps of DNA replication in order?
Replication occurs in three major steps:
the opening of the double helix and separation of the DNA strands, the priming of the template strand, and the assembly of the new DNA segment
. During separation, the two strands of the DNA double helix uncoil at a specific location called the origin.
What is another name for replication fork?
Also known as
helix destabilizing enzyme
. Helicase separates the two strands of DNA at the Replication Fork behind the topoisomerase.
What is the leading strand in DNA replication?
The leading strand is
a single DNA strand
that, during DNA replication, is replicated in the 3′ – 5′ direction (same direction as the replication fork). DNA is added to the leading strand continuously, one complementary base at a time.
What are the enzymes required for DNA replication?
| Important Enzymes in DNA Replication | Enzyme Function | DNA helicase Unwinds the double helix at the replication fork | Primase Provides the starting point for DNA polymerase to begin synthesis of the new strand | DNA polymerase Synthesizes the new DNA strand; also proofreads and corrects some errors |
|---|
Which enzyme is not required for DNA replication?
RNA polymerase
is an enzyme that transcribes RNA from DNA; it is not essential for DNA replication. This enzyme is easy to confuse with primase, whose primary function is to synthesize the RNA primers necessary for replication.
What are the 3 main enzymes?
- amylase and other carbohydrase enzymes break down starch into sugar.
- protease enzymes break down proteins into amino acids.
- lipase enzymes break down lipids (fats and oils) into fatty acids and glycerol.
What is the first step of DNA replication group of answer choices?
Which is the first step of DNA replication?
The DNA double helix begins unwinding
. What causes DNA strands in humans to unzip? An enzyme (helicase) breaks bonds between base pairs.
Where does DNA replication begin and end?
The double-stranded DNA of the circular bacteria chromosome is opened
at the origin of replication
, forming a replication bubble. Each end of the bubble is a replication fork, a Y-shaped junction where double-stranded DNA is separated into two single strands.
What is the first step of DNA replication quizlet?
The first major step for the DNA Replication to take place is
the breaking of hydrogen bonds between bases of the two antiparallel strands
. The unwounding of the two strands is the starting point. The splitting happens in places of the chains which are rich in A-T.
What are the 5 steps in DNA replication?
- Step 1: Replication Fork Formation. Before DNA can be replicated, the double stranded molecule must be “unzipped” into two single strands.
- Step 2: Primer Binding. The leading strand is the simplest to replicate.
- Step 3: Elongation.
- Step 4: Termination.
Where does DNA replication happen?
DNA replication occurs
in the cytoplasm of prokaryotes and in the nucleus of eukaryotes
. Regardless of where DNA replication occurs, the basic process is the same. The structure of DNA lends itself easily to DNA replication. Each side of the double helix runs in opposite (anti-parallel) directions.
What are the 7 steps of DNA replication?
- Initiation.
- Primer Synthesis.
- Leading Strand Synthesis.
- Lagging Strand Synthesis.
- Primer Removal.
- Ligation.
- Termination.
