- Start by rotating the objective lens to lowest power.
- Place a slide on the stage, label side up, with the coverslip centered.
- On LOW POWER ONLY, use the coarse focus knob to get the object into focus.
- If you cannot see anything, move the slide slightly while viewing and focusing.
What are the 10 steps in using microscope?
- Step 1: Moving Your Microscope. Carry the microscope with two hands. …
- Step 2: Microscope Lens Care. Never touch any lens with your fingers. …
- Step 3: Microscope Parts. …
- Step 4: Prepare a Slide. …
- Step 5: Insert the Slide. …
- Step 6: Set Up for Viewing. …
- Step 7: Light Control. …
- Step 8: Focus the Microscope.
What are the steps in focusing on a microscope?
- Start by rotating the objective lens to lowest power.
- Place a slide on the stage, label side up, with the coverslip centered.
- On LOW POWER ONLY, use the coarse focus knob to get the object into focus.
- If you cannot see anything, move the slide slightly while viewing and focusing.
What is the first step in focusing your microscope?
Look through the eyepiece (1) and move the focus knob until the image comes into focus
. Adjust the condenser (7) and light intensity for the greatest amount of light.
What are the 5 steps in focusing on a microscope?
- Plug in the microscope.
- Turn the microscope light on.
- Place the stage as far away from the objective lenses as possible.
- Rotate the revolving nosepiece until the lowest power objective lens clicks into place.
- Looking at your stage from the side, place the specimen over the hole in the stage.
How do you use a light microscope step by step?
- Always start on low power with a clean slide. …
- Centre the slide so the specimen is underneath the objective lens.
- Use the Coarse adjustment knob to obtain a general focus. …
- Use the fine adjustment knob to obtain a clear focus.
- Centre the specimen in the low power field of view before you move to medium power.
What are the basic steps in use and care of microscope?
- Tip 1: Handle with care. …
- Tip 2: Keep lenses clear of slides. …
- Tip3: Clean after using immersion oil. …
- Tip 4: Cover when not in use. …
- Tip 5: Look after the bulb. …
- Tip 6: Store in a clean, dry place. …
- Tip 7: Only use special lens paper or wipes for cleaning the lenses.
What three things change as you increase magnification?
- Change in Magnification. Changing from low power to high power increases the magnification of a specimen. …
- Light Intensity Decreases. The light intensity decreases as magnification increases. …
- Field of View. …
- Depth of Field. …
- Working Distance. …
- Oil Immersion.
What are the three main parts of a compound microscope?
- Head/Body. houses the optical parts in the upper part of the microscope.
- Base. of the microscope supports the microscope and houses the illuminator.
- Arm. connects to the base and supports the microscope head.
Where is the fine focus on a microscope?
The coarse adjustment knob is the bigger of the two knobs and is located closest to the arm of the microscope. The fine adjustment knob is the smaller of the smaller of the two knobs and is
located further away from the arm of the microscope
.
Should you wear gloves when using a microscope?
All glass slides must be disposed of into a sharps container • Immersion oil is an irritant so ensure you wear gloves when handling it.
Wear gloves if using stains
. NB: The microscope should be set up in a laboratory area but away from wet areas such as sinks and staining materials.
How is the beam focused in a light microscope?
Principles. The light microscope is an instrument for visualizing fine detail of an object. It does this by creating a
magnified image through the use of a series of glass lenses
, which first focus a beam of light onto or through an object, and convex objective lenses to enlarge the image formed.
What is the proper way to carry a microscope quizlet?
To carry a microscope,
grip the arm with one hand and place the other hand underneath the base
.
Why would you use a light microscope?
Microscopes are used to produce magnified images. … light microscopes are
used to study living cells
and for regular use when relatively low magnification and resolution is enough. electron microscopes provide higher magnifications and higher resolution images but cannot be used to view living cells.
Why do cells need to be stained prior to microscopy?
The most basic reason that cells are stained is
to enhance visualization of the cell or certain cellular components under a microscope
. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.