A spectrophotometer is an analytical instrument used to quantitatively measure the transmission or reflection of visible light, UV light or infrared light . Spectrophotometers measure intensity as a function of light source wavelength.
What are the types of spectrophotometer and its uses?
Types of Spectrophotometers: UV-VIS Spectrophotometer
UV-VIS spectrophotometer is used to measure the material of absorbance and quantitative analysis at the visible or ultraviolet light (200 ~ 760nm). ... UV spectrophotometer can be divided into single beam, split beam, double beam for different applications.
What are the uses of spectrometer?
- Monitoring dissolved oxygen content in freshwater and marine ecosystems.
- Studying spectral emission lines of distant galaxies.
- Characterization of proteins.
- Space exploration.
- Respiratory gas analysis in hospitals.
What are the uses and functions of spectrometer?
A spectrometer is any instrument used to probe a property of light as a function of its portion of the electromagnetic spectrum , typically its wavelength, frequency, or energy. The property being measured is usually intensity of light, but other variables like polarization can also be measured.
What is the use of spectrophotometer in the laboratory?
Spectrophotometer is a device used to measure the intensity of light as a function of the color of light . Spectrophotometers find greater application in clinical laboratories and other scientific fields such as molecular biology, chemistry and biochemistry.
What is the principle of spectrometer?
Spectrophotometry is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution. The basic principle is that each compound absorbs or transmits light over a certain range of wavelength.
What are the main parts of spectrometer?
- 2.1 Entrance Slit. Light from the source enters the entrance slit and the size of the slit determines the amount of light that can be measured by the instrument. ...
- 2.2 Grating. ...
- 2.3 Detector.
What are the two types of spectrophotometer?
Among the different types of spectrophotometry, there are two primary methods employed; absorption spectrophotometry, which is concerned with the absorption of radiation and specific spectra of light, and Ultraviolet-Visible Range spectrophotometry , which is concerned with the reflectance of specific spectra of a given ...
What are the two basic types of spectrophotometer?
There are two major classes of devices: single beam and double beam . A double beam spectrophotometer compares the light intensity between two light paths, one path containing a reference sample and the other the test sample.
Why is spectrophotometry important?
A spectrophotometer measures the amount of light transmitted through a substance and is an invaluable instrument in science. Without it, determining the chemical make-up of materials would be very difficult and not very accurate.
Who uses spectroscopy?
Spectroscopy is used in physical and analytical chemistry because atoms and molecules have unique spectra. As a result, these spectra can be used to detect, identify and quantify information about the atoms and molecules. Spectroscopy is also used in astronomy and remote sensing on Earth.
What are the types of spectrometer?
- Matter and Energy. Spectrometry is based on interactions between matter and energy. ...
- Colorimeters. In a colorimeter, a sample is exposed to a single wavelength of light, or is scanned with many different wavelengths of light. ...
- UV Spectrometers. ...
- IR Spectrometers. ...
- Atomic Spectrometers. ...
- Mass Spectrometers.
What are the three main components of a spectrophotometer?
A spectrophotometer consists of three primary components: a light source, optics to deliver and collect the light, and a detector .
What is the purpose of a blank cuvette?
Determining blank, or zero, values is an important step in all photometric measurements. It serves the calibration of the photometer , which is thus set to “zero”.
What is beer Lambert law?
What does the Beer’s Law state? Beer’s Law or the Beer-Lambert Law states that the amount of energy absorbed or transmitted by a solution is proportional to the solution’s molar absorptivity and the concentration of solute .
Which light is used in spectrophotometer?
Light source
Two kinds of lamps, a Deuterium for measurement in the ultraviolet range and a tungsten lamp for measurement in the visible and near-infrared ranges , are used as the light sources of a spectrophotometer. A continuous spectrum of 300 – 3,000 nm is emitted.
