Urea agar contains what?
Peptone, glucose, urea, and phenol red
. The pH of the prepared medium is ____ and what color does phenol red turn? During incubation, bacteria possessing urease will produce what?
What is the purpose of protein catabolism Part 1?
Protein catabolism is
the breakdown of proteins into absorbable monomers for further degradation or reassembly
. Protein catabolism in the intestinal lumen is important for several reasons, one of which is mobilizing essential amino acids for absorption.
What chemical compound would you find in the liquid of hydrolyzed gelatin?
In the liquid of hydrolyzed gelatin, you'll find
amino acids
that can be absorbed through the body.
What is the differential ingredient in phenylalanine decarboxylase?
The differential material is
the amino acid phenylalanine
and after incubation a reagent called ferric chloride (FeCl
3
)(10%) is added to detect if the phenylpyruvic acid was produced, or not.
Do all bacteria produce gelatinase?
Do all bacteria produce urease, and gelatinase? How do you know?
No and No bacteria would need
either a particular exoenzyme (gelatinase & urease) to overcome these buffers. Nutrient gelatin can be incubated at 35C.
What enzyme breaks down gelatin?
The enzyme that breaks down the proteins in gelatin is
gelatinase
. (Most enzymes are named after what they do and end in “ase.”) When the enzymes break down the protein, the jiggly block turns into liquid.
Is hydrolyzed collagen same as gelatin?
Sometimes, gelatin is processed further to produce a substance called collagen hydrolysate, which contains
the same amino acids as gelatin
and has the same health benefits. However, it dissolves in cool water and doesn't form a jelly. This means it may be more palatable as a supplement to some people.
What is the function of the enzyme urease?
Urease, an enzyme that
catalyzes the hydrolysis of urea, forming ammonia and carbon dioxide
. Found in large quantities in jack beans, soybeans, and other plant seeds, it also occurs in some animal tissues and intestinal microorganisms.
When canned foods spoil what causes the blackening of the cans?
When canned foods spoil, what causes the blackening of the cans?
Reduction of sulfur in various compounds forms hydrogen sulfide
. The H2S reacts with metal ions (e.g. ferrous ions) to form metal sulfides, which are black.
Is E coli lysine decarboxylase positive?
More striking is the observation that strains of EIEC are almost universally negative for lysine decarboxylase (LDC) activity whereas
almost 90% of normal flora E. coli are positive
.
Is phenylalanine deaminase selective or differential?
| A B | Is the Phenylalanine Deaminase test Differential or Selective? Differential | Is the Bile esculin test Differential or Selective? Both | Describe the procedure of the Citrate Test. Get Simmons Citrate agars and aseptically inoculate with a needle lightly and incubate. |
|---|
What bacteria is phenylalanine deaminase test positive?
When 10% ferric chloride is added to phenylalanine deaminase medium inoculated with
Proteus mirabilis
, the presence of phenylpyruvic acid causes the media to turn dark green. This is a positive result.
What bacteria are gelatinase positive?
Bacillus anthracis, B. cereus
and several other members of the genus are gelatinase-positive, as are Clostridium tetani and perfringens.
Is P vulgaris gelatinase positive?
vulgaris is tested using the API 20E identification system it produces
positive results
for sulfur reduction, urease production, tryptophan deaminase production, indole production, sometimes positive gelatinase activity, and saccharose fermentation, and negative results for the remainder of the tests on the testing …
What are the two end products from the breakdown of gelatin by gelatinase?
Gelatinase allows the organisms that produce it to break down gelatin into
smaller polypeptides, peptides, and amino acids
that can cross the cell membrane and be utilized by the organism. When gelatin is broken down, it can no longer solidify.
Why does pineapple stop Jelly setting?
Jelly contains gelatine which partially consists of protein molecules. … Fresh fruits such a pineapple, kiwi and papaya
contain enzymes which break down these protein molecules, making them smaller
, so they can't tangle up, which stops the jelly setting. This is similar to how the enzymes in your stomach break down food.
