BIOLOG GN plates are increasingly used to
characterize microbial communities by determining the ability of the communities to oxidize various carbon sources
. Studies were done to determine whether the BIOLOG GN plate assay accurately reflects the catabolic potential of the inoculum used.
What is a Biolog plate?
The Biolog AN MicroPlateTM (Figure 1) is designed for identification of a
very wide range of anaerobic bacteria
, including the genera Bifidobacterium, Clostridium, Eubacterium, Fusobacterium, Lactobacillus, Lactococcus, Megasphaera, Pectinatus, Pediococcus, Peptostreptococcus, Propionibacterium and Weissella.
How do Biolog plates work?
Biolog’s carbon source utilization technology
identifies environmental and pathogenic microorganisms
by producing a characteristic pattern or “metabolic fingerprint” from discrete test reactions performed within a 96 well microplate.
What is Biolog in microbiology?
Biolog –
Microbial Identification & Characterization
– Biolog – world leader in cell based technology and assays for microbiology & cell biology using phenotype microarray technology. Explore More. Microbial Identification to Human Cell Analysis. Cell Phenotyping. Biolog.
What is community level physiological profiling?
Community-level physiological profiling (CLPP) is
a technique which offers an easily applied protocol yielding information regarding mixed microbial community function and functional adaptations over space and time
.
What is an EcoPlate?
An EcoPlate is a
96-well microplate that contains 31 common carbon sources from altogether five compound groups
—that is, carbohydrates, carboxylic and ketonic acids, amines and amides, amino acids and polymers—plus a blank well as a control, all these replicated thrice to control variation in inoculum densities.
What is inoculating fluid?
The inoculating fluid contains
a redox dye that turns purple if
the organism is actively metabolizing in the well. … The pattern of respiration is compared to a database to identify the organism.
What is OmniLog?
OmniLog –
Fully Autommatic Bacteria, Yeast and Fungi Identification Systems
.
What does a microplate reader measure?
A microplate reader
detects light signals produced by samples which have been pipetted into a microplate
. … Microplate-based measurements detect light signals produced by a sample, converted by a sample or transmitted through a sample. The signal is measured by a detector, usually a photomultiplier tube (PMT).
What is Biolog?
Biology is
the study of life
. The word “biology” is derived from the Greek words “bios” (meaning life) and “logos” (meaning “study”). In general, biologists study the structure, function, growth, origin, evolution and distribution of living organisms.
What is microbial identification system?
Microbial identification systems play an important role in
environmental monitoring, contamination investigation, and cleanroom control by identifying potentially harmful bacteria, yeast, and fungi
.
What is automated microbial identification system?
It is an
instrument used for automatic computer-assisted identification of bacteria
. It mainly involves staining, motility test, cultural characteristics, a series of biochemical tests. The automatic bacteria identification system automatically identifies the bacteria in very short time.
How is AWCD calculated?
For each soil sample and at each incubation time point, average well color development (AWCD) was calculated according to the equation:
AWCD = [Σ (C – R)] / n where C represents the absorbance value of control wells
(mean of 3 controls), R is the mean absorbance of the response wells (3 wells per carbon substrate), and …
What is the function of inoculating needle?
An inoculation needle is used in
the transfer of microbial organisms from plate culture to needle by first sterilizing the needle to prevent contaminants
. The lid of the agar plate culture is then removed to allow the needle access to the microorganisms cultured on the agar plate.
What is an inoculating loop used for?
Inoculating loops are used
to introduce microorganisms into a culture medium
.
How do you make liquid bacterial culture?
Using a
sterile pipette tip or toothpick
, select a single colony from your LB agar plate. Drop the tip or toothpick into the liquid LB + antibiotic and swirl. Loosely cover the culture with sterile aluminum foil or a cap that is not air tight. Incubate bacterial culture at 37°C for 12-18 hr in a shaking incubator.
