Listen to pronunciation. (LY-sis) In biology, lysis refers
to the breakdown of a cell caused by damage to its plasma (outer) membrane
. It can be caused by chemical or physical means (for example, strong detergents or high-energy sound waves) or by infection with a strain virus that can lyse cells.
What happens during lysis?
Cell lysis or cellular disruption is a method in which
the outer boundary or cell membrane is broken down or destroyed in order to release inter-cellular materials such
as DNA, RNA, protein or organelles from a cell.
What does lysis mean?
Listen to pronunciation. (LY-sis) In biology, lysis refers
to the breakdown of a cell caused by damage to its plasma (outer) membrane
. It can be caused by chemical or physical means (for example, strong detergents or high-energy sound waves) or by infection with a strain virus that can lyse cells.
What is an example of lysis?
Lysis: Destruction. Hemolysis is the destruction of red blood cells with the release of hemoglobin; bacteriolysis is the destruction of bacteria; etc. Lysis can also refer to the subsidence of one or more symptoms of an acute disease as, for example, the
lysis of fever in pneumonia
.
What does lysis mean in surgery?
Lysis of adhesions
is a surgery to cut bands of tissue that form between organs. These bands are called adhesions. They are often caused by scar tissue that formed after an earlier surgery.
What is the purpose of lysis?
The word lysis comes from the greek word for “loosen.” Cell lysis is the process of rupturing the membrane or walls of a cell. The purpose of a cell lysis buffer is
to use a chemical mixture to disrupt the exterior environment of a cell in a way that causes it to break open and release its contents
.
How does lysis occur?
Cytolysis, or osmotic lysis, occurs
when a cell bursts due to an osmotic imbalance that has caused excess water to diffuse into the cell
. Water can enter the cell by diffusion through the cell membrane or through selective membrane channels called aquaporins, which greatly facilitate the flow of water.
How do I check cell lysis?
if you want to monitor lysis, you
centrifuge your samples and analyse protein or DNA content in the supernatant after centrifugation
. The values (A 280 for protein or A260 for nucleic acids should come to a maximum when lysis is complete.
How does the lysis method work?
The technique involves
freezing a cell suspension in a dry ice/ethanol bath or freezer and then thawing the material at room temperature or 37°C
. This method of lysis causes cells to swell and ultimately break as ice crystals form during the freezing process and then contract during thawing.
What causes lysis in bacteria?
Membrane lysis, or rupture, is a cell death pathway in bacteria frequently caused by
cell wall-targeting antibiotics
. Although previous studies have clarified the biochemical mechanisms of antibiotic action, a physical understanding of the processes leading to lysis remains lacking.
What does the root lysis mean?
Lysis:
Destruction
. Hemolysis is the destruction of red blood cells with the release of hemoglobin; bacteriolysis is the destruction of bacteria; etc. Lysis can also refer to the subsidence of one or more symptoms of an acute disease as, for example, the lysis of fever in pneumonia.
What cell causes lysis?
Cell lysis is a common outcome of viral infection. It consists of a disruption of cellular membranes, leading to cell death and the release of cytoplasmic compounds in the extracellular space. Lysis is actively induced by
many viruses
, because cells seldom trigger lysis on their own.
What is a lysis reaction?
Lysis refers to
the breaking down of the cell
, often by viral, enzymic, or osmotic mechanisms that compromise its integrity. A fluid containing the contents of lysed cells is called a “lysate”. … It gently and rapidly dissolves cell membranes at low concentrations without denaturing proteins.
What does the Greek word lysis means?
Physis (/ˈfaɪˈsɪs/; Ancient Greek: φύσις [phýsis]) is a Greek philosophical, theological, and scientific term, usually translated into English—according to its Latin translation “natura”—
as “nature”
. The term originated in ancient Greek philosophy, and was later used in Christian theology and Western philosophy.
Is lysis a root?
scientific/medical word-forming element meaning “loosening, dissolving, dissolution,” from Greek lysis “a loosening, setting free, releasing; dissolution; means of letting loose,” from lyein “to unfasten, loose, loosen, untie,” from PIE root *leu- “to loosen, divide, cut apart.”
Is lysis a root word?
Word origin: from Greek λύσις,
lysis from lyein = to separate
. Related forms: lyse (verb), lytic (adjective). Derived terms: cytolysis, dermatolysis, haemodialysis, haemolysis, enzymolysis, hydrolysis.
