Nuclease-Free Water is used in various molecular biology applications requiring nuclease-free conditions, such as in processing DNA or RNA (i.e. PCR, cDNA synthesis, or qPCR). This product is sterile-filtered (0.2 μm), free of RNase and DNase activity, endotoxin-free, and not DEPC-treated.
What is the purpose of nuclease free water?
Nuclease-free Water is ideal for the preparation of reagents and for use in enzymatic reactions . No toxic agents, such as DEPC, are used in the production of this water, so as to avoid inhibition in enzymatic reactions.
Is nuclease free water the same as DI water?
Nuclease- Free Water is prepared in a proprietary process, which yields DNase, RNase, and nuclease-free, deionized water without the use of chemical additives, such as diethylpyrocarbonate (DEPC). Nuclease-Free Water is provided in nuclease-free containers.
Is nuclease free water distilled?
UltraPure DNAse/RNase-Free Distilled Water.
Is water needed for PCR?
PCR and related PCR-based techniques, including quantitative PCR and reverse transcriptase PCR , require nuclease-free water to avoid the degradation of the nucleic acid. ... In addition to nuclease-free requirement, water should also be free of specific ions, organics and bacteria.
How do you make nuclease free water?
- Get MilliQ (reverse osmosis purified) water. ...
- Add 1 ml DEPC (Diethylpyrocarbonate) per 1000 ml of MilliQ or double distilled water (i.e. to a final concentration 0.1%) and mix thoroughly.
- Let the DEPC-mixed water incubate for 12 hours at 37°C.
- Autoclave DEPC-mixed water for 15 minutes.
Is MilliQ water sterile?
MilliQ water is NOT sterile . Distribution of bacteria within operating laboratory water purification systems.
Is ddH2O nuclease free?
ddH2O (Sterile, Nuclease- Free water) – GI101-01.
What is deionized water?
Deionized Water
DI grade water, or Type II water, is purified water that has had almost all its mineral ions removed , such as cations like sodium, calcium, iron, and copper, and anions like chloride and sulfate.
What is the difference between distilled water and double distilled water?
Distilled water is purified via distillation i.e boiling and then condensation of the water. So, a double distilled water has gone through the process of distillation twice . While mQ water has been deionised/demineralised and gone through filter to remove all life forms or treated with UV-irradiation.
What is the pH of distilled water?
It is the assumption that because distilled water has been purified, it has a neutral pH of 7 .
What is nuclease water?
Nuclease-Free Water is used in various molecular biology applications requiring nuclease-free conditions, such as in processing DNA or RNA (i.e. PCR, cDNA synthesis, or qPCR). This product is sterile-filtered (0.2 μm), free of RNase and DNase activity, endotoxin-free, and not DEPC-treated.
What type of water is used for PCR?
we must use nuclease free water in PCR reaction so as to prevent degredation of DNA. Nuclease free water is used in order to dilute the concentration of the reagents to the proper final concentration.
What is PCR water used for?
Description: PCR-grade Water is intended for use in molecular biology applications including PCR and RT-PCR. The ultra-pure and sterile filtered water is manufactured free of detectable inhibitors, contaminants or enzymatic activity.
Can I use MilliQ water for PCR?
In practice, milliQ water suffices for most standard PCR (you don’t need to buy nuclease free water or DEPC-treat your water). ... If you want to exercise an abundance of caution, use nuclease free water. While DNA can be stored in water, unbuffered water is not great for long-term stability.
Is water for injection RNase free?
Description: Molecular Biology Grade UltraPure Water is free of all RNase enzymes .
