Requirements for DNA Synthesis
There are four basic components required to initiate and propagate DNA synthesis. They are:
substrates, template, primer and enzymes
.
Which one of the following is not a process of DNA replication?
palindromic
is not a process of DNA replication.
Which of the following is not required for DNA replication?
Which of the following proteins is not necessary during DNA replication? Explanation:
RNA polymerase
is an enzyme that transcribes RNA from DNA; it is not essential for DNA replication. This enzyme is easy to confuse with primase, whose primary function is to synthesize the RNA primers necessary for replication.
What are the 5 steps in DNA replication?
- Step 1: Replication Fork Formation. Before DNA can be replicated, the double stranded molecule must be “unzipped” into two single strands.
- Step 2: Primer Binding. The leading strand is the simplest to replicate.
- Step 3: Elongation.
- Step 4: Termination.
What is the first step in DNA replication?
Replication occurs in three major steps: the
opening of the double helix
and separation of the DNA strands, the priming of the template strand, and the assembly of the new DNA segment. During separation, the two strands of the DNA double helix uncoil at a specific location called the origin.
What is the replication of DNA called?
The process of DNA duplication is called
DNA replication
. Replication follows several steps that involve multiple proteins called replication enzymes and RNA. In eukaryotic cells, such as animal cells and plant cells, DNA replication occurs in the S phase of interphase during the cell cycle.
Which enzyme is not required for DNA replication?
RNA polymerase
is an enzyme that transcribes RNA from DNA; it is not essential for DNA replication. This enzyme is easy to confuse with primase, whose primary function is to synthesize the RNA primers necessary for replication.
Where does DNA replication occur?
DNA replication occurs
in the cytoplasm of prokaryotes and in the nucleus of eukaryotes
. Regardless of where DNA replication occurs, the basic process is the same. The structure of DNA lends itself easily to DNA replication. Each side of the double helix runs in opposite (anti-parallel) directions.
What are the 6 steps in DNA replication?
- Recognition of initiation point. …
- Unwinding of DNA – …
- Template DNA – …
- RNA Primer – …
- Chain Elongation – …
- Replication forks – …
- Proof reading – …
- Removal of RNA primer and completion of DNA strand –
What are the six enzymes used in DNA replication?
During DNA replication, one new strand (the leading strand) is made as a continuous piece. The other (the lagging strand) is made in small pieces. DNA replication requires other enzymes in addition to DNA polymerase, including
DNA primase, DNA helicase, DNA ligase, and topoisomerase.
What is DNA replication write down its method?
DNA replication is the process by
which DNA makes a copy of itself during cell division
. … The separation of the two single strands of DNA creates a ‘Y’ shape called a replication ‘fork’. The two separated strands will act as templates for making the new strands of DNA.
What are the 4 steps of replication?
- Step 1: Replication Fork Formation. Before DNA can be replicated, the double stranded molecule must be “unzipped” into two single strands.
- Step 2: Primer Binding. The leading strand is the simplest to replicate.
- Step 3: Elongation.
- Step 4: Termination.
What are the 7 steps of DNA replication?
- Initiation.
- Primer Synthesis.
- Leading Strand Synthesis.
- Lagging Strand Synthesis.
- Primer Removal.
- Ligation.
- Termination.
What is last step in DNA replication?
Step 4:
Termination
Another enzyme called DNA ligase joins Okazaki fragments together forming a single unified strand. The ends of the linear DNA present a problem as DNA polymerase can only add nucleotides in the 5′ to 3′ direction. The ends of the parent strands consist of repeated DNA sequences called telomeres.
What is DNA replication and why is it important?
DNA replication is the process by which a double-stranded DNA molecule is copied to produce two identical DNA molecules. Replication is an essential process because,
whenever a cell divides, the two new daughter cells must contain the same genetic information, or DNA, as the parent cell
.
What are the three major key players in DNA replication?
One of the key players is the enzyme DNA polymerase, also known as DNA pol. In bacteria, three main types of DNA polymerases are known:
DNA pol I, DNA pol II, and DNA pol III
. It is now known that DNA pol III is the enzyme required for DNA synthesis; DNA pol I and DNA pol II are primarily required for repair.
