Wet-mount Slides A wet-mount slide is when the sample is placed on the slide with a drop of water and covered with a coverslip, which holds it in place through surface tension. Advantages – This type of
slide preparation allows you to view microscopic living things without them drying out
.
What are the advantages to using a wet mount slide preparation quizlet?
Wet mount slide preparation
allows the viewing of specimen behavior as they interact with the environment
. Which of the three factors affecting image quality is altered by the light source? Contrast can be altered by light intensity and staining. You just studied 13 terms!
What is the advantage of using a wet mount quizlet?
-the wet mount is
a safer way to view pathogenic microorganisms
. -the specimen can be viewed as living cells. -The motility of a specimen can be viewed under the microscope. -The specimen can be viewed as living cells.
What is the advantage of putting liquid on a specimen as used in wet mount slide?
Wet Or Temporary Mount
A microscope slide using a wet mount provides a number of advantages.
Putting a few drops of water or chemical stain on a specimen makes it more visible under the microscope
. For live specimens, the water will allow the user to view its movement or motility and observe cell division.
What is not an advantage of a wet mount?
Disadvantages: –
Movement of specimens make it difficult to draw/photograph
. -Evaporation of liquid. -Focus-some organisms may swim vertically, moving in and out of focus.
What precautions should be taken when preparing a wet mount?
- use appropriate amount of water or Vaseline.
- no air bubble should be present under the cover slip.
- mount should not be thicker as thicker mounts do not produce clear image of the specimen.
What is a limitation of using a wet mount?
Organisms are killed in
a wet mount therefore motility cannot be ascertained. Organisms are artificially colored therefore you are unable to see them in their natural state. There is very little contrast between the organisms and the background making it difficult to see.
What are the disadvantages of permanent slides?
What are some disadvantages of permanent slides? Permanent slides
contain specimens that are fixed, dehydrated and possibly also microtomed (sliced into thin sections)
. The organisms are therefore not moving. Over time the specimen may also start to lose color.
What are the limitations of preparing wet mount?
Possible problems of making a wet mount. The cover glass floats and moves: This
is due to too much water. Remove water with the help of a tissue paper
. Under no circumstances should there be water droplets on top of the cover glass.
Why a cover slip is used?
When viewing any slide with a microscope, a small square or circle of thin glass called a coverslip is placed over the specimen.
It protects the microscope and prevents the slide from drying out when it’s being examined
. The coverslip is lowered gently onto the specimen using a mounted needle .
What types of organisms can be viewed on a wet mount?
Yeast cells are large enough to be seen at 400X magnification. The largest bacteria, such as Bacillus megaterium, can also be seen at 400X. Most other bacteria require 1000X magnification.
Algae and protozoa
are best viewed in a wet mount without staining.
Why is a wet preparation discarded in disinfectant solution?
Why is a wet preparation discarded in disinfectant solution or biohazard container? Wet preparation is discarded in disinfectant solution or biohazard container
to kill the infectious living organisms
. This is for laboratory safety reasons because wet mount contain living organisms in them.
What lens could get dirty with oil if you move the revolving nosepiece in the wrong direction?
What lens could get dirty with oil if you move the revolving nosepiece in the wrong direction after viewing under oil immersion? add a drop of immersion oil before rotating
the 100x lens
into position.
What is meant by wet mount?
Medical
Definition
of
wet mount
: a glass slide holding a specimen suspended in a drop of liquid (as water) for microscopic examination also : a specimen
mounted
in this way.
Does a wet mount requires the addition of certain dyes?
A wet mount requires the addition of certain dyes and cell fixatives
approximately 12 hours before viewing
the specimen. oil immersion increases the resolving power of the specimen.
Is a wet mount safer?
The wet mount is
a safer way to view pathogenic organisms
. The specimen can be viewed as living cells. The contrast of a specimen is enhanced with the addition of water to the slide.
