What Is The Correct Order Of Draw In Phlebotomy?

by | Last updated on January 24, 2024

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If just a routine coagulation assay is the only test ordered, then

a single light blue top tube may be drawn

. If there is a concern regarding contamination by tissue fluids or thromboplastins, then one may draw a non-additive tube first, and then the light blue top tube.

What is the standard order of draw?

The order of draw is based on

CLSI Procedures and Devices

for the Collection of Capillary Blood Specimens; Approved Standard – Sixth Edition, September 2008. This standard recommends that EDTA tubes be drawn first to ensure good quality specimen, followed by other additive tubes and finally, serum specimen tubes.

What is the order of blood collection tubes?

Standard order of draw:

BLOOD CULTURES, royal blue, red, light blue, SST (Gold), green, tan, yellow, pink, pearl, lavender

. If a coag tube (light blue) is the only tube or the first tube to be drawn, a 5 mL discard tube must be drawn first. or incubation conditions. incubation may be required.

What are the steps in phlebotomy?

  1. Assemble equipment. …
  2. Identify and prepare the patient. …
  3. Select the site. …
  4. Perform hand hygiene and put on gloves. …
  5. Disinfect the entry site. …
  6. Take blood. …
  7. Fill the laboratory sample tubes. …
  8. Draw samples in the correct order.

Why is order of draw important in phlebotomy?

The order of draw is recommended for both glass and plastic venous collections tubes when drawing multiple specimens for medical laboratory testing during a single venipuncture. The purpose is

to avoid possible test result error due to additive carryover

. All additive tubes should be filled to their stated volumes.

Why is the order of collection so important?

Order of draw is

essential in avoiding cross contamination from additives of one tube to the next

. Cross contamination, depending on the tests ordered, could cause seriously erroneous results, improper patient treatment and possibly death. While most blood collections (straight venipuncture vs.

What is the best website to draw blood from an infant?


The neonatal heel prick, also known as a dermal puncture

, is by far the most popular way to collect blood from newborns and infants up to about six months of age. Dermal punctures are preferable because, when done correctly, they are guaranteed to produce blood, removing the uncertainty of needle sticks.

How do you remember the order of draw?


A mnemonic device

is a phrase or saying that is easy to remember and relate back to in the future. It’s a great way to remember order of draw (OOD) because you can modify the sentence to be most helpful to you. In this example, the tubes that make the order of draw are identified by color.

What happens if the proper order of draw is not followed?

In the era of lyophilized anticoagulants, order of draw is no longer important.

Contamination of serum samples with K EDTA

will occur routinely if order of draw is not followed. During syringe collections, contamination with K EDTA may rarely occur if order of draw is not followed.

What are the 3 main veins to draw blood?

The most site for venipuncture is the antecubital fossa located in the anterior elbow at the fold. This area houses three veins:

the cephalic, median cubital, and basilic veins

(Figure 1).

What are the four phlebotomy techniques?

Four different ways of taking blood were observed:

cannulation and a syringe

(38%), cannula with evacuated tube and adaptor (42%), syringe and needle into vein (14%) and evacuated tube system used conventionally (6%).

What is the correct way to end a venipuncture procedure?

  1. Determine that the patient is in no distress.
  2. Thank the patient.
  3. Replace any items that were relocated during the collection.
  4. Gather all equipment and supplies.

What is the most common complication of phlebotomy?


Hematoma

: The most common complication of phlebotomy procedure.

Which blood culture bottle do you draw first?


The blue (aerobic) blood culture bottle should be filled first

, then the purple (anaerobic) bottle as the butterfly tubing may contain air. Air entering the purple bottle will impede the growth of anaerobic organisms.

Why is it hard to draw my blood?

If you have been to a clinic or lab before and had the phlebotomist stick you more than once for a blood draw, you may have been told that you are a “difficult stick.” This can happen to people for quite a few different reasons, including small or deep veins, rolling veins,

dehydration

, collapsing veins, constricted …

What can I expect from a phlebotomy class?


Draw blood

from patients and blood donors. Talk with patients and donors to help them feel less nervous about having their blood drawn. Verify a patient’s or donor’s identity to ensure proper labeling of the blood. Label the drawn blood for testing or processing.

Maria LaPaige
Author
Maria LaPaige
Maria is a parenting expert and mother of three. She has written several books on parenting and child development, and has been featured in various parenting magazines. Maria's practical approach to family life has helped many parents navigate the ups and downs of raising children.