Pure broth cultures contain a liquid media, while slant cultures
contain a semi-solid media
. The substance used to make the media of a slant culture…
How is the inoculation of a broth tube and a slant tube different?
How is the inoculation of a broth tube and a slant tube different?
Open tubes should be held at an angle instead of straight up
. What is the most important reason for this action?
What is the difference between a broth and a slant quizlet?
A broth is liquid,
whereas a slant is solid
. Select the inoculating tool that is more appropriate to remove bacterial growth from a liquid culture.
What is the difference between the slant tube and the stab tube?
Slant tubes (see Fig. 8A)are tubes containing a nutrient medium plus a solidifying agent, agar-agar. … Stab tubes (deeps) are tubes of hardened agar medium which are inoculated by “stabbing” the inoculum into the agar (see Fig. 9).
What inoculation technique is used for slant tubes?
epidermidis plate to your sterile tube. Use the
fishtail inoculation
technique if you have the slant.
What is the difference between a slant and a slant deep?
If the medium in the test tube is allowed to
harden
in a slanted position, the tube is designated an agar slant; if the tube is allowed to harden in an upright position, the tube is designated an agar deep tube; and if the agar is poured into a Petri dish, the plate is designated an agar plate.
What is a slant tube used for?
Agar slants can be used to
culture bacterial cells for identification
. Attempting to identify bacteria from a large sample is difficult because bacteria are small and can be difficult to find.
How do you inoculate a slant quizlet?
How does one inoculate an agar slant? To inoculate an agar slant
obtain a inoculating loop with specimen then proceed to spread lightly without puncturing the agar from bottom of the agar
(bottom of the test tube) to the top of the agar (opening of test tube.)
What does it mean to inoculate quizlet?
Inoculation.
A process for growing a specific organism for studying to increase its visibility
, so that it is possible to manage and handle for analysis. Inoculation involves. using a sterile tool to spread the sample on the surface of a solid medium or to introduce the sample into a flask or tube.
What does inoculated mean in microbiology?
2a :
to introduce microorganisms or viruses onto
or into (an organism, substrate, or culture medium) inoculated a rat with bacteria. b : to introduce (as a microorganism or antiserum) into an organism or onto a culture medium inoculate a pure culture of bacteria into a healthy host.
How is the inoculation of a broth tube and a slant tube different quizlet?
How is the inoculation of a broth tube and a slant tube different?
Open tubes should be held at an angle instead of straight up
. What is the most important reason for this action?
What are inoculation techniques?
Inoculation method can affect symptom development. Typically, inoculation is performed via
mechanical wounding or grafting
. Mechanical inoculation includes cutting, slashing, and rubbing, and is the only procedure for fulfilling Koch’s postulates.
How do you inoculate a broth tube?
Pick up a tube of sterile nutrient broth with your free hand, carefully remove the cap (cotton plug) with your little finger of the hand holding the loop, and flame the mouth of the tube. Insert the
loop into
the sterile broth and inoculate it by gently moving the loop back and forth in order to disperse the cells.
What do we use to inoculate our agar plates and liquid broth?
Inoculating loops and needles made of metal wire
can be used to transfer bacteria from one medium to another, such as from the surface of an agar plate to a broth. Metal tools may be sterilized by heating them in the flame of a Bunsen burner.
When transferring from a broth to a solid why flame the top of the broth tube?
Aseptic technique
is used to prevent environmental bacteria (e.g., from the air) from contaminating cultures. This is why we flame the mouths of the culture tubes before and after transferring bacteria. You just studied 32 terms!
What is the difference between a petri dish and a slant?
Agar slants contain the same medium as petri plates
, but in a tube in which the agar has solidified while the tube is on a slanted surface.
What is the difference between a broth culture and a broth medium?
Broth cultures are liquid cultures used to grow bacteria in laboratories. To create a broth culture, a scientist begins with a
sterile liquid growth
medium. The medium is inoculated with bacteria and placed in an incubator at the appropriate temperature.
What is the best definition of slant?
1 :
to give an oblique or sloping direction
to. 2 : to interpret or present in line with a special interest : angle stories slanted toward youth especially : to maliciously or dishonestly distort or falsify.
What is a slant and deep in microbiology?
SLANT:
solid medium made with agar and various nutrients and indicators
. Slanting gives the bacteria a greater surface area on which to grow in a tube. … Bacteria are inoculated onto a slant using a loop and grow in the surface of the agar. SLANT/DEEP: solid medium made with agar and various nutrients and indicators.
Why are agar slants better?
Why are agar slants better suited than agar plates to maintain stock cultures? Slants are better suited
because they can be capped, preventing the agar and culture from drying out
. The cap also prevents airborne contaminates from entering the slant. Slants take up less storage space.
What do you need to do to this slant before inoculating it with cells?
Inoculate the slant by
transferring cells with an inoculating loop
from a single-colony microorganism on a plate to the slant’s surface. Move the loop across the surface of the slant and recap the tubes. Incubate the slant until there is evidence of growth, then put the tube in a refrigerator.
What type of inoculation tool is used to inoculate a broth?
An inoculation needle
is used in inoculating a sterile broth culture.
Why did we use an inoculating needle to inoculate the agar deep tube?
Explain why a straight inoculating needle is used to inoculate an agar deep tube?
So the penetration is smooth and small and won’t allow oxygen in deep
. Called a stab inoculation.
What is inoculation and incubation performed?
Inoculation is
the process of introducing microorganisms or suspension of microorganisms into a culture medium
. Incubation is the process of allowing inoculated microorganisms to grow under required growing conditions.
What inoculated media?
In microbiology, inoculation is defined as
introducing microorganisms into a culture where they can grow and reproduce
. More generally, it can also be defined as introducing a certain substance into another substance. … You can inoculate bacteria and other microorganisms into a variety of media where they will grow.
What is the term for the plates slants or broths used to grow bacteria?
Broth- Slants- Plates or
petri dishes
– Nutrients in broth/agar used to culture microorganisms(bacteria)
How is inoculation done in microbiology?
In Microbiology, inoculation refers to
the act of introducing micro-organisms or suspensions of microorganisms (e.g. bacteria into a culture medium)
. … A sterile inoculation loop or pipette is placed in bacterial culture and then either spread on an agar plate or mixed into liquid media/broth.
Which of the following is the proper technique for inoculating an agar slant with a broth culture?
Which of the following is the proper technique for inoculating an agar slant?
Streak the surface of the media with a back-and-forth motion using an inoculating loop
.
What is slant and stab?
slant culture one made on the surface of solidified medium in a tube which has been tilted to provide a greater surface area for growth. … stab culture a culture into which the
organisms
are introduced by thrusting a needle deep into the medium.
How is inoculation different from vaccination?
Vaccination is the use of vaccines to give you immunity to a disease. Immunization is the process that happens in your body. Usually that’s due to vaccination but it can come from other exposure to a pathogen. Inoculation has come to mean the same thing
as immunization
.
Is there a difference between a vaccine and an inoculation?
Of the three words,
vaccinate is the most narrow
because it specifically means to give a vaccine to someone. Inoculate is more general and can mean to implant a virus, as is done in vaccines, or even to implant a toxic or harmful microorganism into something as part of scientific research.
What causes pellicle formation on a broth?
Motile bacteria (those with flagella) can swim. Their movement will create a uniform cloudiness (turbidity) in the broth.
Non-motile bacteria with waxy cell walls tend to float
at the surface of the broth, producing a surface membrane called a pellicle.
Which of the following is a common reason to inoculate a culture onto a slant rather than into a broth?
Which of the following is a common reason to inoculate a culture onto a slant rather than into a broth?
To avoid the contamination that is common from broth splashing around.
When transferring a bacterial colony is it best to use a loop or a needle does it make any difference?
Terms in this set (152) Would you use an inoculating loop to transfer bacteria to an agar deep tube? No,
a needle would be best since it will do the least amount of damage to the agar and allow it to reseal around the inserted bacteria
.
What is inoculation chamber?
Used for transference of medium and inoculation
in a micro-organism free environment, inoculation chambers are made of thick ply board on top and bottom surface a plywood sheet on the back.
What is inoculation in fermentation?
The fermentation process involves an inoculum development step, which is the
preparation of a population of microorganisms from a stock dormant culture
to a state useful for inoculating a final production fermenter.
What are the types of inoculation?
Different types of inoculation media are
agar plates, broth culture, slant culture, plate culture, stab culture, etc.
, which has been discussed in detail below.
