A chromatography detector is a device used in gas chromatography (GC) or liquid chromatography (LC)
to detect components of the mixture being eluted off the chromatography column
. There are two general types of detectors: destructive and non-destructive.
What are the detectors in gas chromatography?
The FID
is the most common detector used in gas chromatography. The FID is sensitive to, and capable of detecting, compounds that contain carbon atoms (C), which accounts for almost all organic compounds.
What is an ideal detector in gas chromatography?
An ideal GC detector is distinguished by several characteristics. The first requirement is
adequate sensitivity to provide a high resolution signal for all components in the
mixture. … Moreover, the detector should be reliable, predictable and easy to operate.
How do GC detectors work?
An
FID uses a flame to ionize organic compounds containing carbon
. … Following separation of the sample in the GC column, each analyte passes through a flame, fuelled by hydrogen and zero air, which ionises the carbon atoms.
What is the basic principle of gas chromatography?
Principle of gas chromatography:
The sample solution injected into the instrument enters a gas stream which transports the sample into a separation tube known as the “column.”
(Helium or nitrogen is used as the so-called carrier gas.) The various components are separated inside the column.
Which detector is not used in GC?
Explanation:
UV visible spectrometric detector
is not used in gas chromatography.
Which detector is used in HPLC?
- UV-Vis Detectors. The SPD-20A and SPD-20AV are general-purpose UV-Vis detectors offering an exceptional level of sensitivity and stability. …
- Refractive Index Detector. …
- Fluorescence Detectors. …
- Evaporative Light Scattering Detector. …
- Conductivity Detector.
How do you identify compounds in gas chromatography?
THE identification of unknown compounds separated by gas chromatography is often effected by comparison of their retention values with those of known compounds together with
infra-red spectro-photometry or mass spectrometry
.
How do you read a gas chromatography?
- The X-Axis: Retention Time. Usually, the x-axis of the gas chromatogram shows the amount of time taken for the analytes to pass through the column and reach the mass spectrometer detector. …
- The Y-Axis: Concentration or Intensity Counts. …
- Differences in Gas Chromatogram Models.
What is the most sensitive GC detector?
Electron capture detector, (ECD)
. The most sensitive detector known. Allows for the detection of organic molecules containing halogen, nitro groups etc.
What is the difference between GC FID and GC MS?
GC-FID is
also considered more sensitive, more reproducible and covers a wider dynamic range when compared to GC-MS in full scan monitoring mode (10). … However, none of these analyses were performed using HRMS equipped with both FID and MS detectors that used the same samples at the same time.
Is GC quantitative or qualitative?
Gas chromatography (GC) can be
used for both qualitative and quantitative analyses
. This chapter begins with a brief look at qualitative analysis. The chromatographic parameter used for qualitative analysis is the retention time or some closely related parameter.
Why oxygen is not used in GC?
Whenever gases is used in the chromatography process, there's a potential for gas leaks, whether from the supply lines, storage tanks, or from the chromatograph itself.
Nitrogen gas displaces oxygen
. If nitrogen were to leak, air levels would become deficient of oxygen and employees could suffer health problems.
What are the main parts of gas chromatography?
It consists of three main components: 1) an injector, which is a port meant for injecting the samples into the GC, 2) a column in which the analyte gets separated into individual components, depending upon its affinity with the stationary phase and the mobile carrier gas phase, and 3) the detector, where the …
What is the mechanism of gas chromatography?
Gas chromatography is the
process of separating compounds in a mixture by injecting a gaseous or liquid sample into a mobile phase, typically called the carrier gas, and passing the gas through a stationary phase
. The mobile phase is usually an inert gas or an unreactive gas such as helium, argon, nitrogen or hydrogen.
Which detector is not suitable in HPLC?
A UV detector
cannot be used with solvent which has UV absorbance. Sometimes the organic solvent used for GPC analysis absorbs UV, and thus UV detector cannot be used. It provides a direct relationship between the intensity and analyte concentration.
