Do not bump, jar, or move the centrifuge while
the rotor is spinning
. Instruct all other persons in the area to stay clear of the unit while it’s operating. Make sure you don’t have the cord dangling from a table edge where someone could catch their foot in it and pull down the centrifuge.
Which of the following are important safety considerations for using a centrifuge?
- Use the centrifuge on a level surface.
- Check the unit before use.
- Inspect tubes before use.
- Check the maximum load tolerance and speed.
- Balance tubes correctly.
- Keep the lid closed during cycles.
- Monitor the centrifuge.
- Shut down in event of noise or vibrations.
What is very important to remember when using a centrifuge?
If the liquid has a higher or lower density than water, you
must balance the tubes by mass
, not volume. The total mass of each tube should be as close as possible – this becomes increasingly important at very high rotor speeds. Running a centrifuge with unbalanced load could permanently damage the centrifuge.
What is the most important part of loading a centrifuge?
Tip 3—
Balance the weight
within the centrifuge—Balancing is a critical detail before starting a centrifuge run. It is best to have perfect balance within the centrifuge. If the balance is off, the centrifuge can experience violent shaking, which can harm the sensitive mechanical parts within.
What is the importance of a centrifuge and why is it important?
Centrifuges are
most often used to separate different liquids and solid particulates from liquids
, but they may be used for gases. They are also used for purposes other than mechanical separation.
How do you use a centrifuge safety?
- Use safety cups whenever possible.
- Disinfect or clean weekly and after all spills or breakages.
- Lubricate O-rings and rotor threads weekly.
- Do not operate the centrifuge without the rotor properly balanced.
- Do not use rotors that have been dropped.
What happens when you don’t balance the centrifuge?
Centrifuges, which operate at high speed, have great potential for injuring users if not operated properly.
Unbalanced centrifuge rotors can result in injury or death
. Sample container breakage can release aerosols that are harmful if inhaled.
What are 5 safety precautions for a laboratory?
- Know locations of laboratory safety showers, eyewashstations, and fire extinguishers. …
- Know emergency exit routes.
- Avoid skin and eye contact with all chemicals.
- Minimize all chemical exposures.
- No horseplay will be tolerated.
- Assume that all chemicals of unknown toxicity are highly toxic.
What is the function of centrifuge?
A centrifuge is
used to separate particles suspended in a liquid according to particle size and density, viscosity of the medium, and rotor speed
. Within a solution, gravitational force will cause particles of higher density than the solvent to sink, and those less dense than the solvent to float to the top.
What is the principle and application of centrifugation?
Definition of Centrifugation
It is a technique which involves
the application of centrifugal force to separate particles from a solution according to their size, shape, density, the viscosity of the medium and rotor speed
.
What is the principle of centrifugation?
A centrifuge works by using the principle of sedimentation:
Under the influence of gravitational force (g-force), substances separate according to their density
. Different types of separation are known, including isopycnic, ultrafiltration, density gradient, phase separation, and pelleting.
What happens if you spin blood too soon?
Failure to adhere to these wait periods may result in
fibrin clots forming within the serum phase of the centrifuged sample
, which may require additional handling to rim the clot and may introduce sample contamination.
When operating the centrifuge students should do which of the following?
When operating the centrifuge, students should do which of the following? Make sure that the lid is completely shut and the safety knob is turned to engage the safety switch.
Centrifuge for approximately 30 seconds
.
Why is it important to centrifuge blood?
Centrifuge Promptly
It is important
to separate the cellular and liquid portions of a blood specimen
as soon as possible when the test requires a sample of serum or plasma. This is because the cells interact with the serum/plasma, altering its chemical composition and affecting test results.
How is centrifugation used in everyday life?
Some common examples of centrifugation include:
The extraction of fat from milk in order to produce skimmed milk
. The removal of water from moist lettuce with the help of a salad spinner. The Spin-drying of water in washing machines in order to remove water from the clothing.
Why do we use centrifugation techniques?
Centrifugation is a technique used
for the separation of particles from a solution according to their size, shape, density, viscosity of the medium and rotor speed
. The particles are suspended in a liquid medium and placed in a centrifuge tube. … Centrifugation is making that natural process much faster.
