Replica plating is the technique by which each colony/clone is inoculated onto multiple plates according to a numbered scheme. This method
allows each clone to be tested by a variety of methods
, while retaining a master plate from which clones can be picked.
What is true of replica plating quizlet?
Replica plating is
a transfer technique that may be used to produce multiple copies of a particular colonial arrangement on a number of agar plates
. … Replica plating may be used to test the ability of a variety of bacteria to grow on a number of different types of media.
What is replica plating used for?
The purpose of replica plating is
to be able to compare the master plate and any secondary plates, typically to screen for a desired phenotype
.
Why velvet cloth is used in replica plating?
Velvet Pad is a consumable that is used with the Replica Plating Apparatus and
allows of colony transfer from Agar Plate while retaining the colony orientation
. Used for screening auxotrophy and antibiotic resistance.
What is plating method in microbiology?
This technique typically is used
to separate microorganisms contained within a small sample volume
, which is spread over the surface of an agar plate, resulting in the formation of discrete colonies distributed evenly across the agar surface when the appropriate concentration of cells is plated.
What is meant by replica plating?
Replica plating is the
technique by which each colony/clone is inoculated onto another plate according to a numbered scheme
. This method allows each clone to be tested by a variety of methods, while retaining a master plate from which clones can be picked.
What is pour plate technique?
Pour plate method is usually the
method of choice for counting the number of colony-forming bacteria present in a liquid specimen
. Because the sample is mixed with the molten agar medium, a larger volume can be used than with the spread plate. … Each colony represents a “colony-forming unit” (CFU).
Is replica plating useful for identifying Auxotrophs?
Identifying Bacterial Mutants
One common technique used to identify bacterial mutants is called replica plating. This technique is used to detect nutritional mutants, called auxotrophs, which have a mutation in a gene encoding an enzyme in the biosynthesis pathway of a specific nutrient, such as an amino acid.
What can you conclude from the Mega plate experiment quizlet?
What can you conclude from Mega-plate experiment?
Bacteria evolve from low resistance mutant to moderately resistant before finaly gave rise to highly resistant strains
. In theory, daughter cells of replicating bacteria have genetically identical chromosomes from the parent cell.
Which change in a gene’s DNA sequence would have the least effect?
| Question Answer | Which change in a gene’s DNA sequence would have the least effect on the eventual amino acid sequence produced from it? addition/deletion of 3 consecutive nucleotides | Chemical mutagens often act by altering the hydrogen bonding properties of the nucleobase. |
|---|
What does countable plate mean?
Countable plates
measure colony forming units of bacteria which are in range between 30 and 300 colonies
. The dilution of sample which is used in countable plate is roughly 1 / 10 dilution of sample.
How would you isolate a mutant from the replica plate?
The standard approach to isolation of such mutants is
replica plating of a library of mutagenized bacteria on a control plate and a plate with a subinhibitory concentration of an antibiotic, followed by identification of colonies that grow only on the control plate
.
What is gradient plate technique?
The technique is termed the gradient. plate method and can be performed easily by. anyone who can grow bacterial cultures. The method involves
solidification of a
.
layer of agar medium while the petri dish
is.
What are the different types of plating?
There are three popular plating styles:
classic, free form, and landscape
.
What is the difference between pour plate and spread plate method?
The key difference between Pour plate and Spread plate is that
a known volume of the sample is spread on the surface of the agar medium in spread plate
, while, in pour plate, a known volume of the sample is mixed with agar and then poured into a plate.
What are the advantages and disadvantages of spread plating?
- The optimum method for aerobes while microaerophilic tends to grow slower.
- The dilutions should be Accurate.
- The volume of inoculum greater than 0.1 mL on the nutrient agar not soak well and may result in colonies to coalesce as they form.
