2. Apply a small amount of lens cleaner (70% isopropyl alcohol) to one end of a piece of lens paper or clean swab. Holding the dry end, gently drag the moist end of the lens paper or swab across the front of the objective/ocular several times using a fresh section of moist lens paper each time.
What is used to clean the microscope lenses?
To clean microscope eyepiece lenses, breathe condensation onto them and then wipe them with lens tissue. Kim-wipes are made by Kleenex and generally will work well. For stubborn spots, wipe the surface with tissue moistened with 95% alcohol. Wipe the lens dry with a dry tissue.
Why microscope lenses should not be touched?
Never touch the lenses with your fingers. Your body produces an oil that smudges the glass . This oil can even etch the glass if left on too long. Use only LENS PAPER to clean the glass.
What is the safest solvent for cleaning an objective lens?
Acetone is the safest solvent for cleaning an objective lens. Only lint-free, optically safe tissue should be used to wipe off microscope lens. ... Once the focus is achieved at one magnification, a higher-power objective lens can be rotated into position without fear of striking the slide.
What should you use to clean the lenses of a microscope quizlet?
Xylene – is a solvent used with lens paper to clean grease and oil from the lenses.
Which is the only objective lens that can come in contact with oil?
Before using immersion oil, make sure that your 100x objective lens is made for use with immersion oil. Begin by focusing your sample using the 40x objective lens.
Which lens must cleaned last and why?
If your eyepiece lens turns, turn it and see if the spot moves as well. If so, the dirt is on the eyepiece lens (if not, the dirt is internal and you should probably have it cleaned by a professional). If you only see it at one power, the dirt is most likely on that particular objective lens.
What 3 things do you do when you are finished using your microscope?
- First step. Turn light off and allow microscope to sit for five minutes.
- Second step. Turn stage all the way down.
- Third Step. return objectives to low magnification.
- Fourth Step. Remove slide and rest stage clips on. ...
- Fith Step. Loosely wrap and secure cord.
- Sixth Step. ...
- Seventh step. ...
- eight step.
What is the rule for touching lenses?
What is the rule for touching lenses? Do not touch lenses with fingers , or leave liquids on objective lenses. After use, return the objective to low power, remove the slide and if necessary wipe clean the stage, then put it all back!
What is the advantage of using a wet mount?
Wet-mount Slides A wet-mount slide is when the sample is placed on the slide with a drop of water and covered with a coverslip, which holds it in place through surface tension. Advantages – This type of slide preparation allows you to view microscopic living things without them drying out .
Which focus knob is used to raise and lower the stage?
Coarse focus : The large coarse focus knob is used to raise or lower the stage until the image is in focus.
Which is the shortest objective lens?
A scanning objective lens that magnifies 4x is the shortest objective and is useful for getting a general overview of a slide. A low-power objective lens magnifies 10x, but remember that it is coupled with an eyepiece lens, so the total magnification is 10x times the power of the eyepiece lens.
Why do not we use alcohol to clean the lenses instead of xylene?
For the Cargille Type A or B immersion oil, you can use naphtha, xylene, or turpentine (use very small amounts on lens tissue). Do not use water, alcohol or acetone, as the oil is insoluble to these solvents . ... If it is not cleaned well, the residual oil will crystallize on the lens.
How do you properly clean a microscope quizlet?
- turn the light control all the way down.
- turn the microscope off.
- rotate the nosepiece to the 4x objective. ...
- drop the stage all the way down using the coarse focus knob.
- remove your microscope slide from the stage. ...
- using a dry piece of lens paper, wipe the 40x objective first in a circular motion.
When should you use the scanning lens?
When should you use the scanning lens on the microscope? Whenever a new slide is viewed or when the view of the specimen in the field of view of a higher-power lens is lost .
Which items are appropriate to use when cleaning your objective lenses?
General procedure for cleaning an objective:
70% ethanol on cotton-tipped applicator . Sparkle on cotton tipped applicator. Distilled water (or breathe on the objective), wipe gently with lens paper.
