When focusing on a slide, ALWAYS start with either the 4X or 10X objective . Once you have the object in focus, then switch to the next higher power objective. Re-focus on the image and then switch to the next highest power.
When using a microscope what do you do first?
When you begin using the biological compound microscope, always begin with the 4x objective lens (the lowest magnification). This will allow you to focus on your sample and help prevent you from smashing your objective lens into the stage.
Why do I need to start with the lowest magnification setting when using a microscope?
When using a light microscope it’s important to start with the low power objective lens as the field of view will be wider, increasing the number of cells you are able to see . This makes it easier to find what you’re looking for.
How do you start a microscope?
- Turn the revolving turret (2) so that the lowest power objective lens (eg. ...
- Place the microscope slide on the stage (6) and fasten it with the stage clips.
- Look at the objective lens (3) and the stage from the side and turn the focus knob (4) so the stage moves upward.
What to do after using microscope?
After using the microscope, turn off the illuminator and wait for it to cool for several minutes before putting it away . By allowing the bulb to cool you will extend its life. Also, make sure you do not store your microscope in an area that has corrosive chemical fumes that can destroy lenses or metal parts.
What is the advantage of using a wet mount?
Wet-mount Slides A wet-mount slide is when the sample is placed on the slide with a drop of water and covered with a coverslip, which holds it in place through surface tension. Advantages – This type of slide preparation allows you to view microscopic living things without them drying out .
What three things change as you increase magnification?
This change alters the magnification of a specimen, the light intensity, area of the field of view, depth of field, working distance and resolution .
What part of the microscope makes the image clearer?
Condensers are lenses that are used to collect and focus light from the illuminator into the specimen. They are found under the stage next to the diaphragm of the microscope. They play a major role in ensuring clear sharp images are produced with a high magnification of 400X and above.
What can you see at 100x magnification?
At 100x magnification you will be able to see 2mm . At 400x magnification you will be able to see 0.45mm, or 450 microns. At 1000x magnification you will be able to see 0.180mm, or 180 microns.
What are the 10 steps in using microscope?
- Step 1: Moving Your Microscope. Carry the microscope with two hands. ...
- Step 2: Microscope Lens Care. Never touch any lens with your fingers. ...
- Step 3: Microscope Parts. ...
- Step 4: Prepare a Slide. ...
- Step 5: Insert the Slide. ...
- Step 6: Set Up for Viewing. ...
- Step 7: Light Control. ...
- Step 8: Focus the Microscope.
What are the 5 steps in focusing on a microscope?
- Plug in the microscope.
- Turn the microscope light on.
- Place the stage as far away from the objective lenses as possible.
- Rotate the revolving nosepiece until the lowest power objective lens clicks into place.
- Looking at your stage from the side, place the specimen over the hole in the stage.
What 3 things do you do when you are finished using your microscope?
- First step. Turn light off and allow microscope to sit for five minutes.
- Second step. Turn stage all the way down.
- Third Step. return objectives to low magnification.
- Fourth Step. Remove slide and rest stage clips on. ...
- Fith Step. Loosely wrap and secure cord.
- Sixth Step. ...
- Seventh step. ...
- eight step.
What are the 3 tips for using the microscope?
- Tip 1: Handle the microscope with care. ...
- Tip 2: Choose a proper place to work with your microscope. ...
- Tip 3: Do not let your lens kiss the slide. ...
- Tip 4: Clean immediately after using immersion oil. ...
- Tip 5: Only use special lens paper or wipes to clean the lenses. ...
- Tip 6: Cover the microscope when not in use.
What part of the microscope are the objectives attached to?
Nosepiece : The upper part of a compound microscope that holds the objective lens. Also called a revolving nosepiece or turret.
What precautions should be taken when preparing a wet mount?
- use appropriate amount of water or Vaseline.
- no air bubble should be present under the cover slip.
- mount should not be thicker as thicker mounts do not produce clear image of the specimen.
