Alfred Hershey was a phage geneticist who, with his research assistant, Martha Chase, did one of the most famous experiments in molecular biology. The “blender” experiment proved that DNA carried genetic information.
Who were Alfred Hershey and Martha Chase?
Alfred Hershey was a phage geneticist who, with his research assistant, Martha Chase, did one of the most famous experiments in molecular biology. The “blender” experiment proved that DNA carried genetic information.
What did Hershey-Chase discover?
The Hershey-Chase experiment, which demonstrated that the genetic material of phage is DNA, not protein . The experiment uses two sets of T2 bacteriophages. In one set, the protein coat is labeled with radioactive sulfur ( 35 S), not found in DNA.
What did Griffith Avery Hershey and Chase do?
Groundbreaking experiments by Griffith, Avery, Hershey, and Chase disproved the notion that proteins were genetic material . ... Together, these experiments demonstrated that DNA was transferred between generations and that this molecule had the ability to transform the properties of a cell.
How did Hershey and Chase label DNA?
Method. Hershey and Chase used T2 phage , a bacteriophage. The phage infects a bacterium by attaching to it and injecting its genetic material into it. They labeled the phage DNA with radioactive Phosphorus-32.
Did Martha Chase get a Nobel Prize?
Martha Chase was an American geneticist. In 1952, during the “Hershey-Chase Experiments,” she accompanied Alfred Hershey, helping to experimentally confirm that DNA, not protein, is the genetic material of life [1]. This discovery was awarded the Nobel Prize in Physiology or Medicine in 1969, yet excluded Chase [2].
Why did Hershey and Chase use a blender?
They infected one sample with radioactive phosphorus-labeled phages, and the other sample with radioactive sulfur-labeled phages. Then, they stirred each sample in a Waring Blender, which was a conventional kitchen blender. They used a blender because centrifuges spun too fast and would destroy the bacterial cells .
What would Hershey and Chase have concluded if both radioactive?
What would Hershey and Chase have concluded if both radioactive 32 P and 35 S were found in the bacteria in their experiment? The virus’s protein coat was not injected into the bacteria . The virus’s DNA was not injected into the bacteria. ... purines in DNA is much greater than the percentage of pyrimidines.
What did Avery conclude caused transformation?
What did Avery conclude caused transformation? DNA was the transforming factor . The harmless bacteria would not have been transformed, and the mice would have lived.
What did Avery conclude?
What did Avery conclude? He concluded that DNA transmits genetic information .
What was the conclusion of Griffith’s experiment?
Griffith concluded that something in the heat-killed S bacteria ‘transformed’ the hereditary properties of the R bacteria . The nature of this ‘transforming principle’ was unknown.
How did Hershey and Chase show that DNA is passed to new phages in phage reproduction?
How did Hershey-Chase show that DNA is passed to new phages in phage reproduction? Hershey and Chase used the radioactive isotope P32 to and S 35 to demonstrate that DNA is passed to new phage particles during phage reproduction.
How did the results of the Hershey-Chase experiment strengthen Avery’s conclusion?
How did the results of the Hershey-Chase experiment strengthen Avery’s conclusions? At the end of this experiment, they found phosphorus-32 in the bacteria , which supports Avery’s theory that genes are located in the DNA of the cell.
What are the 3 roles of DNA?
DNA now has three distinct functions— genetics, immunological, and structural —that are widely disparate and variously dependent on the sugar phosphate backbone and the bases.
Which bacteria killed the mice in Griffith’s experiment?
As part of his experiments, Griffith tried injecting mice with heat-killed S bacteria (that is, S bacteria that had been heated to high temperatures, causing the cells to die). Unsurprisingly, the heat-killed S bacteria did not cause disease in mice.
Is carbon a DNA?
A single basic unit or “building block” of DNA consists of a sugar , a phosphate group and a base. Sugars are rings of carbon and oxygen atoms. The sugar in DNA has 5 carbon atoms (labelled 1′ – 5′), and is called deoxy-ribose (hence the “Deoxy-ribo” in DNA).
