These are classified into six types: (1) Basal media, (2) Enriched media, (3) Selective (4) Indicator media, (5) Transport media, and (6) Storage media . 1. BASAL MEDIA. Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media.
What are the 3 types of media used in a microbiology lab?
These are classified into six types: (1) Basal media, (2) Enriched media, (3) Selective (4) Indicator media, (5) Transport media, and (6) Storage media . 1. BASAL MEDIA. Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media.
Why do we use media in lab?
Types of Media Used in the Laboratory
The media used in the laboratory have to be chosen to suit the nutritional requirements of the species of organism to be grown . Isolation from a mixture can sometimes be facilitated by the use of media designed for a special purpose.
What are the 3 types of culture media based on consistency?
Based on consistency culture media is classified as liquid, semi-solid and solid media .
What is the purpose of selective media?
Selective and differential media are used to isolate or identify particular organisms . Selective media allow certain types of organisms to grow, and inhibit the growth of other organisms. The selectivity is accomplished in several ways.
What are examples of selective media?
- Eosin methylene blue contains dyes that are toxic for Gram-positive bacteria. ...
- YM (yeast extract, malt extract agar) has a low pH, deterring bacterial growth.
- MacConkey agar is for Gram-negative bacteria.
- Hektoen enteric agar is selective for Gram-negative bacteria.
What is pure culture media?
A pure culture is usually derived from a mixed culture (one containing many species) by transferring a small sample into new, sterile growth medium in such a manner as to disperse the individual cells across the medium surface or by thinning the sample manyfold before inoculating the new medium. ...
Why do we sterilize culture media?
When microbiological media has been made, it still has to be sterilized because of microbial contamination from air, glassware, hands, etc . Within a few hours there will be thousands of bacteria reproducing in the media so it has to be sterilized quickly before the microbes start using the nutrients up.
Why do we need culture media?
Culture media is of fundamental importance for most microbiological tests: to obtain pure cultures , to grow and count microbial cells, and to cultivate and select microorganisms. ... A microbiological culture medium is a substance that encourages the growth, support, and survival of microorganisms.
Why do we prepare culture media?
Culture media are used for quality control tests of nonsterile raw materials and finished products as well as for microbial contamination (sterility) tests in applications such as hygiene monitoring, sterilization process validation and determination of the effectiveness of preservatives and antimicrobial agents.
What are the five main ingredients of culture media?
- FORMULATION OF CULTURE MEDIA. ...
- 1 Nutrients: proteins/peptides/amino-acids.
- 2 Energy: carbohydrates.
- 3 Essential metals and minerals: calcium, magnesium, iron, trace metals: phosphates, sulphates etc.
- 4 Buffering agents: phosphates, acetates etc.
What are the examples of culture media?
- Tryptic Soy Agar (TSA) Tryptic Soy Agar – uninoculated. ...
- MacConkey (lactose) Agar. MacConkey Agar – uninoculated. ...
- Eosin-methylene Blue Agar (EMB) Eosin Methylene Blue Agar – uninoculated. ...
- Hektoen Agar. Hektoen – uninoculated. ...
- Mannitol Salt Agar. ...
- Triple Sugar Iron Agar (TSI)
What are the common ingredients of culture media?
Some of the ingredients of culture media include water, agar, peptone, casein hydrolysate, meat extract, yeast extract and malt extract . Classification: Bacterial culture media can be classified in at least three ways; Based on consistency, based on nutritional component and based on its functional use.
Why MacConkey agar is selective?
This medium is both selective and differential. The selective ingredients are the bile salts and the dye, crystal violet which inhibit the growth of Gram-positive bacteria . MacConkey agar is commonly used to differentiate between the Enterobacteriaceae. ...
Is chocolate agar selective or differential?
Chocolate agar, a differential medium for gram-positive cocci.
What is the difference between selective and nonselective media?
Selective media generally selects for the growth of a desired organism , stopping the growth of or altogether killing non-desired organisms. Differential media takes advantage of biochemical properties of target organisms, often leading to a visible change when growth of target organisms are present.
