Allowing the tip of the pipet to rise above the liquid in the container usually causes the liquid to be sucked into the pipet bulb. A broken or chipped pipet can reduce the amount of liquid held after transfer . This causes too much liquid to be delivered.
Can a pipette be used without a tip?
It might seem like common sense, but do not aspirate liquid into a pipette without a pipette tip attached . This will not only ruin your results but inevitably damage the interior of the pipette.
Why is it important to change the pipette tip?
Pipette tips can also be a potential source of leachables— trace amounts of chemicals originating from materials or the process equipment that can contaminate the samples. ... Always change the pipette tip after each sample .
Why should you not use wet pipette?
Failure to Pre-Wet Pipette Tip
If pipette operators do not pre-wet the pipette tip prior to initial delivery, sample volume can be lost due to evaporation within the tip .
What should you never do with a pipette?
- Forgetting your head. When using a pipette, always use a tip. ...
- The wrong guy for the job. Make sure your tip is the right one for the volume you are pipetting. ...
- Pushing it too far. ...
- It’s a pipette, not a plunger. ...
- Leaving it lying around. ...
- Using it as a backscratcher. ...
- Holding it wrong. ...
- Dialing it up.
Can you reuse pipette tips when handling DNA?
No! but yes you can reuse tips after proper washing and sterilization for general microbiology work. Nothing can be said about number of times, it depends on material of tips or handling. 3.. Sterlize in autoclave and re use it.
Are all pipette tips sterile?
Pipette tips come in three different types including non-sterile, pre-sterilized and filtered tips . The most commonly used type of pipette tip is non-sterile tips. ... On the other hand, pre-sterilized pipette tips are designed to prevent contamination. They are certified free of DNA, RNase, ATP and pyrogens.
What is the function of pipette?
About Pipettes. A pipette is a laboratory instrument used to measure out or transfer small quantities of liquid , in volumes of milliliters (mL), microliters (μL).
Why is it important to make sure the lid is securely attached to a centrifuge rotor?
Why is it important to make sure the lid is securely attached to a centrifuge rotor? It avoid the loud noise and aerosols . If not attached securely, can come off and damage the centrifuge.
How do you sterilize pipette tips?
- Open the lid of the tip tray a tad—propping it up with the lid’s locking tab helps; a little tape can hold it in place—don’t wrap the box in foil!
- Run your dry cycle as normal.
- Let the damp tips dry in the warm autoclave (or transfer to a drying cabinet/incubator)
How accurate is a pipette?
Micropipettes are now a staple in laboratories and offer accuracy of within a few percent points, usually <3% of the desired measurement .
How do you know if you should expel the last drop of liquid from a volumetric pipette?
As you should (hopefully) know, when you are using a volumetric pipette, you should let the liquid drain out of the bottom and there will be a little left in the tip because of the surface tension. But you should NOT squeeze out that last drop no matter how tempting it may be!
What is the largest source of pipetting problems?
Human error is the largest source of pipetting problems, followed by liquids sticking to the tips, and loss of accuracy when working with viscous liquids (multi-option select question, chart depicts the percent of survey respondents who experienced these various pipetting errors).
Is it safe to fill a pipette by suction?
Since pipettes are glass, you can snap the pipette in two as you force it into the bulb and subsequently drive the broken portion into your hand. Use great care when inserting a pipette into a bulb. If possible, use a suction device where the pipette rests up against it to seal rather than being fully inserted.
When should you use a pipette?
- pipette with a slow, smooth action;
- hold the pipette vertically when drawing liquid in;
- immerse the tip only slightly when drawing liquid in—otherwise, you’ll coat the tip with liquid, which will be transferred along with the volume inside the pipette;
How do you make a pipette harmful material?
Safe Work Practices for Pipetting
Drain a pipette with tip against the inner wall of the receiving vessel . Never forcibly expel any hazardous material from a pipette. Carefully eject the disposable pipette tips to minimize aerosol formation.
