confocal microscopes

Why Is Confocal Microscopy Better Than Fluorescence Microscopy?

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Why Is Confocal Microscopy Better Than Fluorescence Microscopy? Confocal microscopy offers several distinct advantages over traditional widefield fluorescence microscopy, including the ability to control depth of field, elimination or reduction of background information away from the focal plane (that leads to image degradation), and the capability to collect serial optical … What is the difference

Why Do You Use Kohler Illumination?

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Why Do You Use Kohler Illumination? The primary advantage of Köhler illumination is the uniform illumination of the sample. This reduces image artifacts and provides high sample contrast. Uniform illumination of the sample is also critical for advanced illumination techniques such as phase contrast and differential interference contrast microscopy. What is the point of Köhler