What Is Nuclease Free Water? Nuclease-Free Water is used in various molecular biology applications requiring nuclease-free conditions, such as in processing DNA or RNA (i.e. PCR, cDNA synthesis, or qPCR). This product is sterile-filtered (0.2 μm), free of RNase and DNase activity, endotoxin-free, and not DEPC-treated. What is the purpose of nuclease free water? Nuclease-free
What Is The Purpose Of The Proofreading Function Of DNA Polymerase? DNA polymerase proofreading is a spell-checking activity that enables DNA polymerases to remove newly made nucleotide incorporation errors from the primer terminus before further primer extension and also prevents translesion synthesis. Which DNA polymerase is used in proofreading? In bacteria, all three DNA polymerases
Does Nucleotide Excision Repair Require Atp? Abstract. Nucleotide excision repair in eubacteria is a process that repairs DNA damages by the removal of a 12-13-mer oligonucleotide containing the lesion. Recognition and cleavage of the damaged DNA is a multistep ATP-dependent reaction that requires the UvrA, UvrB and UvrC proteins. Is base excision repair ATP dependent?