- Eosin methylene blue contains dyes that are toxic for Gram-positive bacteria. ...
- YM (yeast extract, malt extract agar) has a low pH, deterring bacterial growth.
- MacConkey agar is for Gram-negative bacteria.
- Hektoen enteric agar is selective for Gram-negative bacteria.
What is a selective media?
Selective media are used to select for the growth of a particular “selected” microorganism . For example, if a certain microbe is resistant to aparticular antibiotic (e.g., novobiocin), then that antibiotic can be added to the medium in order to. S. aureus is seen producing yellow colonies on mannitol salt agar.
What are examples of differential media?
- Blood agar (used in strep tests), which contains bovine heart blood that becomes transparent in the presence of hemolytic.
- Streptococcuseosin methylene blue (EMB), which is differential for lactose and sucrose fermentation.
Which of the following is not an example of selective media?
Which of the following is not an example of selective media? Explanation: Tryptic Soy is an example of broth which is used in broth formula. Mannitol agar, MacConkey’s agar, crystal violet blood agar, Thayer Martin agar, etc.
What is an example of a selective and differential media that can be used to identify different Staphylococcus isolates?
Mannitol Salt Agar (MSA) is a selective medium for the isolation of pathogenic and non-pathogenic Staphylococci. Growth of most other organisms is inhibited by a high salt content (7.5% NaCl). MSA also acts as a differential medium for the differentiation of organisms that can ferment mannitol from those that cannot.
Is MacConkey selective or differential?
MacConkey agar is a selective and differentiating agar that only grows gram-negative bacterial species; it can further differentiate the gram-negative organisms based on their lactose metabolism.
Is EMB a selective media?
Eosin methylene blue agar (EMB) is a selective and differential medium used to isolate fecal coliforms. Eosin Y and methylene blue are pH indicator dyes which combine to form a dark purple precipitate at low pH; they also serve to inhibit the growth of most Gram positive organisms.
What is difference between selective and differential media?
Selective and Differential Media. Selective media allows for the growth of specific organisms, while differential media is used to distinguish one organism from another .
Is chocolate agar selective or differential?
Chocolate Agar is an enriched general- purpose medium that supports the growth of most fastidious and non-fastidious organisms. Because it is a non-selective medium , resident flora from clinical specimens may overgrow potential fastidious pathogens, such as Neisseria species.
What is meant by differential media?
Differential media contain compounds that allow groups of microorganisms to be visually distinguished by the appearance of the colony or the surrounding media , usually on the basis of some biochemical difference between the two groups.
What Bacteria grows on Mueller Hinton agar?
Mueller-Hinton agar is a microbiological growth medium that is commonly used for antibiotic susceptibility testing, specifically disk diffusion tests. It is also used to isolate and maintain Neisseria and Moraxella species .
Which of the following describes selective media?
What is the definition of selective media? Selective media incorporate chemical substances that inhibit the growth of one type of bacteria while permitting the growth of another .
What is selective media used for?
Selective and differential media are used to isolate or identify particular organisms . Selective media allow certain types of organisms to grow, and inhibit the growth of other organisms.
What makes MacConkey Agar both selective and differential?
This medium is both selective and differential. The selective ingredients are the bile salts and the dye, crystal violet which inhibit the growth of Gram-positive bacteria. The differential ingredient is lactose.
Is pea selective or differential?
PEA agar is a selective medium that permits the growth of gram-positive cocci while inhibiting most gram-negative organisms.
What are the three main types of microbiological culture media?
Liquid, semisolid and solid media are routinely used for growth of micro-organisms.
