The EnterotubeTM II is an example of a rapid, multi test system used
in identification of unknown oxidase- negative, gram- negative, rod shaped bacteria of the family Enterobacteriaceae
. It consists of a tube with a flat side and contains 12 compartments for different biochemical tests.
What group of organisms is the Enterotube useful for in identifying?
Abstract. Enterotube, a multiple-test system which combines nine biochemical tests useful in the identification of members of
the family Enterobacteriaceae
, was tested and compared with the PathoTec test system in the identification of gram-negative bacilli isolated from clinical specimens.
What is the purpose of using Enterotube?
The Enterotube system provides
a simple, reliable, and rapid method for the presumptive identification of Enterobacteriaceae
. The major advantage of the Enterotube is that all tests are done simultaneously by inoculation from a single isolated colony.
What does Enterotube II test for?
The Enterotube II is a compartmental plastic tube containing 12 media to test for:
acid and gas production from glucose, lysine decarboxylase, ornithine decarbox- ylase, H2S, indole, acetoin
(VP test), phenylalanine de- aminase, urease, citrate utilization, and acid production from adonitol, lactose, arabinose, …
What are the advantages of EnteroPluri test?
EnteroPluri-Test
makes possible the identification of the Enterobacteriaceae and other gram negative, oxidase negative bacteria isolated from clinical and environmental samples
. The identification is based on biochemical tests performed on culture media containing specific substrates.
How do I read my Enterotube results?
The
presence of acetoin, a positive
result, is indicated by the development of a red color within 20 minutes, a negative reaction will appear colorless or light amber. The use of Barritt’s reagent in the VP chamber will be performed after all the results are read for the other chambers in the enterotube.
What is API testing in microbiology?
The analytical profile index or API is
a classification of bacteria based on biochemical tests, allowing fast identification
. This system is developed for quick identification of clinically relevant bacteria. … A bacterial suspension is used to rehydrate each of the wells and the strips are incubated.
How is Enterotube used to identify microorganisms?
The EnterotubeTM II is an example of a rapid, multi test system used in identification of
unknown oxidase- negative, gram- negative, rod shaped bacteria of the family Enterobacteriaceae
. It consists of a tube with a flat side and contains 12 compartments for different biochemical tests.
How do I use API 20e?
Add sterile oil into the ADH, LDC, ODC, H2S and URE compartments. Put some
drops of
water in the tray and put the API Test strip and close the tray. Mark the tray with identification number (Patient ID or Organism ID), date and your initials. Incubate the tray at 37
o
C for 18 to 24 hours.
How do you become a VP test?
ər/ or VP is a test
used to detect acetoin in a bacterial broth culture
. The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth which has been inoculated with bacteria. A cherry red color indicates a positive result, while a yellow-brown color indicates a negative result.
Why is it necessary to have a living specimen for the Enterotube but not for PCR?
Why is a pure culture necessary for biochemical tests such as the Enterotube, but not for DNA probes? …
You use the Enterotube test to find one unknown microorganism
; therefore, if you don’t use a pure culture, you don’t know for sure what microorganism you identified with your results.
Is E coli A Enterobacteriaceae?
Enterobacteriaceae are
a large family of Gram-negative bacteria
that includes a number of pathogens such as Klebsiella, Enterobacter, Citrobacter, Salmonella, Escherichia coli, Shigella, Proteus, Serratia and other species. … These pathogens can also cause healthcare-associated infections (HAIs).
Why are the Enterobacteriaceae of medical significance?
Some members of the Enterobacteriaceae have had a large impact on infectious diseases, clinical microbiology and public health. They are
involved in diarrhoeal diseases
and recognised as one of the major bacterial food-borne pathogens.
What is the main advantage of using the API test?
The major core advantage of API testing is that
it provides access to applications without users actually having to interact with a potentially disparate system
. This helps the tester to detect and recognize the errors early, instead of them becoming larger issues during GUI testing.
How is the EnteroPluri test inoculated?
Inoculate EnteroPluri-Test by
first twisting wire, then withdrawing wire through all twelve compartments applying a turning motion Reinsert wire into EnteroPluri
-Test, using a turning motion through all twelve compartments, until the notch on the wire is aligned with the opening of the tube.
What is an API E test strip?
This API-20E test strip (from bioMerieux, Inc.) is
used to identify the enteric gram negative rods
(although API makes a variety of other test strips for yeast, Staph, anaerobes, etc.) 20 separate test compartments are on the strip, all dehydrated. A bacterial suspension is used to rehydrate each of the wells.
