The term cell line refers
to the propagation of culture after the first subculture
. … It is possible to select a particular cell lineage by cloning or physical cell separation or some other selection method. Such a cell line derived by selection or cloning is referred to as cell strain.
What are cell lines used for?
Cell lines have revolutionized scientific research and are being used in
vaccine production
, testing drug metabolism and cytotoxicity, antibody production, study of gene function, generation of artificial tissues (e.g., artificial skin) and synthesis of biological compounds e.g., therapeutic proteins.
What is cell line and its types?
Continuous immortalized cell lines are comprised of a single cell type that can be serially propagated in culture either for a limited number of cell divisions (approximately thirty) or otherwise indefinitely. Cell lines of a finite life are usually
diploid
and maintain some degree of differentiation.
What is cell line in one word?
Cell Line. A cell line is
a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space
. Lines differ from cell strains in that they become immortalized.
What are types of cell lines?
- Frog cell lines.
- Hamster cell lines.
- Mouse cell lines.
- Rat cell lines.
- Dog cell lines.
What is the cell line?
Cell line. (Science: cell culture) a cell line is
a permanently established cell culture that will proliferate indefinitely given appropriate fresh medium and space
. lines differ from cell strains in that they have escaped the Hayflick limit and become immortalised.
What is a cell?
In biology,
the smallest unit that can live on its own
and that makes up all living organisms and the tissues of the body. A cell has three main parts: the cell membrane, the nucleus, and the cytoplasm. The cell membrane surrounds the cell and controls the substances that go into and out of the cell.
How do you maintain cell lines?
Many continuous mammalian cell lines can be maintained on a relatively simple medium such as
MEM supplemented with serum
, and a culture grown in MEM can probably be just as easily grown in DMEM or Medium 199. However, when a specialized function is expressed, a more complex medium may be required.
How do you develop cell lines?
Cell line development requires
the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein
. The first step in the process is the isolation of single, viable cells. Limiting dilution is a technique that relies on statistical probability but is time consuming.
Can cell lines become immortal?
Changes from nonimmortal origins. While immortalised cell lines often originate from a well-known tissue type,
they have undergone significant mutations to become immortal
.
What is the difference between a cell and cell line?
Primary cell culture is the culture of cells directly isolated from parental tissue of interest; whereas cell line is the culture of cells originated from a primary cell culture, which is generally used to expand cell population and prolong life span. … However, cell lines have
prolonged lifespan
.
What is cell line in microbiology?
Cell lines are
cultures of animal cells that can be propagated repeatedly and sometimes indefinitely
. They arise from primary cell cultures. Primary cultures are initiated directly from the cells, tissues, or organs of animals and are typically used in experiments within a few days.
What is continuous cell line?
Continuous cell lines are
subcultured indefinitely in glass, plastic surfaces, or suspensions
as they are obtained usually from carcinogenic cells. These cell lines are used most often because of faster and longer growth, less nutritional requirement, and high plating efficiency.
How do you identify cell lines?
Cell lines can be tested and identified using
multiallelic variable number of tandem repeats (VNTR)
. HLA typing and DNA fingerprinting using short tandem repeat (STR) and a variable number of tandem repeats for intra-species cross-contamination have been used for cell line identification.
How are cell lines named?
Subclones are named by their parental cell line
, followed by a hyphen and the subclone number (Table 3). … Furthermore, the standardised name provides information about a cell line’s provenance as well as allowing traceability and comparability of the nomenclature-specific information.
What are human cell lines?
Human cell lines are
immortalized cells propagated in vitro from primary explants of human tissue or body fluid
. The use of human cell cultures as a model for more complex biological systems is an integral part of molecular biology, and biomedical research.
